10.1371/journal.pgen.1006291.g006 Vincent Portegijs Vincent Portegijs Lars-Eric Fielmich Lars-Eric Fielmich Matilde Galli Matilde Galli Ruben Schmidt Ruben Schmidt Javier Muñoz Javier Muñoz Tim van Mourik Tim van Mourik Anna Akhmanova Anna Akhmanova Albert J. R. Heck Albert J. R. Heck Mike Boxem Mike Boxem Sander van den Heuvel Sander van den Heuvel T168 and T181 mutants show normal subcellular LIN-5 localization. Public Library of Science 2016 CDK 1-cyclin B Controls Mitotic Spindle Positioning polarity kinase PKC -3 phosphorylates LIN casein kinase 1. GSK 3 phosphorylation vivo phosphorylated LIN GPR residues form part acid binding site mitotic spindle segregates vivo phosphorylated residues cyclin-dependent kinase consensus site chromosome segregation CRISPR 2016-10-06 17:30:02 Figure https://plos.figshare.com/articles/figure/T168_and_T181_mutants_show_normal_subcellular_LIN-5_localization_/3998358 <p>Immunohistochemical staining of embryos heterozygous for <i>egfp</i>::<i>lin-5</i> and wild type or phosphomutant <i>lin-5</i> as indicated. L4 animals were treated with feeding RNAi against control or <i>egfp</i> RNAi to specifically remove <i>egfp</i>::<i>lin-5</i> function, 48 hours before fixation of their embryos. Representative fluorescence microscopy images of embryos stained with anti-LIN-5 (red) and anti-GFP (green) antibodies, and DAPI to visualize DNA. All images taken with same exposure time, objective and magnification. Anterior to the left, scale bars 10 μm.</p>