10.1371/journal.pgen.1006291.g006
Vincent Portegijs
Vincent
Portegijs
Lars-Eric Fielmich
Lars-Eric
Fielmich
Matilde Galli
Matilde
Galli
Ruben Schmidt
Ruben
Schmidt
Javier Muñoz
Javier
Muñoz
Tim van Mourik
Tim
van Mourik
Anna Akhmanova
Anna
Akhmanova
Albert J. R. Heck
Albert
J. R. Heck
Mike Boxem
Mike
Boxem
Sander van den Heuvel
Sander
van den Heuvel
T168 and T181 mutants show normal subcellular LIN-5 localization.
Public Library of Science
2016
CDK 1-cyclin B
Controls Mitotic Spindle Positioning
polarity kinase PKC -3 phosphorylates LIN
casein kinase 1.
GSK 3 phosphorylation
vivo phosphorylated LIN
GPR
residues form part
acid binding site
mitotic spindle segregates
vivo phosphorylated residues
cyclin-dependent kinase consensus site
chromosome segregation
CRISPR
2016-10-06 17:30:02
Figure
https://plos.figshare.com/articles/figure/T168_and_T181_mutants_show_normal_subcellular_LIN-5_localization_/3998358
<p>Immunohistochemical staining of embryos heterozygous for <i>egfp</i>::<i>lin-5</i> and wild type or phosphomutant <i>lin-5</i> as indicated. L4 animals were treated with feeding RNAi against control or <i>egfp</i> RNAi to specifically remove <i>egfp</i>::<i>lin-5</i> function, 48 hours before fixation of their embryos. Representative fluorescence microscopy images of embryos stained with anti-LIN-5 (red) and anti-GFP (green) antibodies, and DAPI to visualize DNA. All images taken with same exposure time, objective and magnification. Anterior to the left, scale bars 10 μm.</p>