Axonal degeneration in the spinal cord of <i>Irp2</i>-null mice. Young JeongSuh R. CrooksDaniel Wilson-OllivierreHayden C. GhoshManik SougratRachid LeeJaekwon CoopermanSharon MitchellJames B. BeaumontCarole A. RouaultTracey 2011 <p>A; Toluidine blue staining of Epon-embedded spinal cord cross-sections at 12 months. Red-circled areas indicate where myelin dense body (MDBs) were found. The yellow star represents the area enlarged in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025404#pone-0025404-g001" target="_blank">Figure 1B</a>. Scale bar = 200 µm. B; Ventral white matter (L4) from wildtype (a), <i>Irp1<sup>+/+</sup>;Irp2<sup>-/-</sup></i> (b), <i>Irp1<sup>+/-</sup>;Irp2<sup>-/-</sup></i> (c) mice at 12 months. Arrows indicate MDBs. Scale bars = 50 µm, 20 µm (inset). C; Quantification of MDBs per spinal cord sections at 4, 7.5, 12 months. The bar chart show average±SEM, n = 4-5, **; p<0.001, analyzed by two-way ANOVA. A summary of the pairwise comparisons is presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025404#pone.0025404.s006" target="_blank">Table S1</a>. D; Progressive accumulation of MDBs in ventral white matter of spinal cords at 4, 7.5 months. Scale bars = 10 µm.</p>