Localization of 45S rDNA by FISH in diploid (a-g) and dihaploid (d-n). Hong Lan Chun-Li Chen Yin Miao Chang-Xiu Yu Wen-Wu Guo Qiang Xu Xiu-Xin Deng 10.1371/journal.pone.0151512.g003 https://plos.figshare.com/articles/figure/Localization_of_45S_rDNA_by_FISH_in_diploid_a-g_and_dihaploid_d-n_/3961260 <p>a and h, red hybridization signals of 45S rDNA on interphase nucleus stained by DAPI; b and i, red Cy3 as hybridization signals of 45S rDNA on metaphase chromosomes stained by DAPI; c and j, DAPI and CMA staining chromosomes (B, Bf, D and Df indicate the type of each chromosome); d, f, k and m, enlarged images of chromosome with fragile site; e, g, i and n, linescan curve analysis of fluorescence signals for the chromosomes with 45S signals. The horizontal axis is the length of one chromosome, and the vertical axis is the gray level which measures the intensity of the fluorescent dye and signals. The red line represents the hybridization site and signal intensity, and the blue line shows chromosomes stained by DAPI. The yellow arrows indicate proximal decondensed 45S rDNA loci and the red arrows indicate the subterminal decondensed 45S rDNA loci, while white arrows indicate condensed 45S rDNA loci. Bar scales = 5 μm.</p> 2016-03-15 00:23:50 chromosomal characteristics 45 S rDNA gene distended 45 S rDNA regions rDNA Citrus sinensis chromosomes CMA 45 S rDNA FISH mapping 45 S rDNA regions NOR PMC site Valencia pollen mother cells DAPI