Non-Leukemogenic Forms of ICN Fail to Rescue 8946 Cell Line Growth and do not Transactivate <i>Myc</i>. Jon C. Aster Nick Bodnar Lanwei Xu Fredrick Karnell John M. Milholland Ivan Maillard Gavin Histen Yunsun Nam Stephen C. Blacklow Warren S. Pear 10.1371/journal.pone.0025645.g006 https://plos.figshare.com/articles/figure/_Non_Leukemogenic_Forms_of_ICN_Fail_to_Rescue_8946_Cell_Line_Growth_and_do_not_Transactivate_Myc_/396051 <p>(A) Comparison of rescue of 8946 cells by various ICN polypeptides following doxycycline-mediated withdrawal of <i>MYC</i>. 8946 cells were transduced with MigRI viruses, sorted for GFP positivity, and then treated with doxycycline (20 microgram/ml) for up to 8 days. Cell numbers at each time point are shown. (B, C) 8946 cells transduced as in (A) were harvested after 24 hr of treatment with doxycycline (20 microgram/ml) or vehicle (PBS). Levels of <i>Myc</i> (B) and <i>Deltex1</i> (C) mRNAs were determined by qRT-PCR.</p> 2011-10-13 01:40:51 forms icn 8946 transactivate