Pal, Sangita Graves, Hillary Ohsawa, Ryosuke Huang, Ting-hsiang Wang, Pingping Harmacek, Laura Tyler, Jessica Commercial H3 K56Ac antibodies are non specific in flies. <p>The top schematic explains the mosaic nature of the wild type histone expression, indicated by GFP staining. All cells express H3 K56R, but the black patches express no endogenous wild type histones. Below are shown wing imaginal discs from flies with clones of cells expressing only H3 K56R (indicated by white arrows in the top row) that are marked by lack of GFP expression. Staining with the indicated H3 K56Ac antibodies showed no difference between H3 K56R mutant clones or the adjacent wild type clones, indicating that the antibodies are non specific. Representative images are shown.</p> Measure H 3 K 56 Acetylation;Commercial Antibodies Widely;yeast;H 3-YFP fusions;K 56Q mutation;FRAP;histone H 3 lysine 56;region;gene;use H 3 K 56Ac;chromatin disassembly dynamics;histone chaperone ASF 1A;function;histone post-translational modifications;H 3 K 56Q mutation;H 3 K 56 acetylation;H 3 K 56R;histone H 3 disassembly;transcriptional induction;chromatin disassembly;H 3 K 56Ac 2016-05-17
    https://plos.figshare.com/articles/figure/Commercial_H3_K56Ac_antibodies_are_non_specific_in_flies_/3939069
10.1371/journal.pone.0155409.g003