Commercial H3 K56Ac antibodies are non specific in flies.
Sangita Pal
Hillary Graves
Ryosuke Ohsawa
Ting-hsiang Huang
Pingping Wang
Laura Harmacek
Jessica Tyler
10.1371/journal.pone.0155409.g003
https://plos.figshare.com/articles/figure/Commercial_H3_K56Ac_antibodies_are_non_specific_in_flies_/3939069
<p>The top schematic explains the mosaic nature of the wild type histone expression, indicated by GFP staining. All cells express H3 K56R, but the black patches express no endogenous wild type histones. Below are shown wing imaginal discs from flies with clones of cells expressing only H3 K56R (indicated by white arrows in the top row) that are marked by lack of GFP expression. Staining with the indicated H3 K56Ac antibodies showed no difference between H3 K56R mutant clones or the adjacent wild type clones, indicating that the antibodies are non specific. Representative images are shown.</p>
2016-05-17 21:14:18
Measure H 3 K 56 Acetylation
Commercial Antibodies Widely
yeast
H 3-YFP fusions
K 56Q mutation
FRAP
histone H 3 lysine 56
region
gene
use H 3 K 56Ac
chromatin disassembly dynamics
histone chaperone ASF 1A
function
histone post-translational modifications
H 3 K 56Q mutation
H 3 K 56 acetylation
H 3 K 56R
histone H 3 disassembly
transcriptional induction
chromatin disassembly
H 3 K 56Ac