10.1371/journal.pone.0155409.g003 Sangita Pal Sangita Pal Hillary Graves Hillary Graves Ryosuke Ohsawa Ryosuke Ohsawa Ting-hsiang Huang Ting-hsiang Huang Pingping Wang Pingping Wang Laura Harmacek Laura Harmacek Jessica Tyler Jessica Tyler Commercial H3 K56Ac antibodies are non specific in flies. Public Library of Science 2016 Measure H 3 K 56 Acetylation Commercial Antibodies Widely yeast H 3-YFP fusions K 56Q mutation FRAP histone H 3 lysine 56 region gene use H 3 K 56Ac chromatin disassembly dynamics histone chaperone ASF 1A function histone post-translational modifications H 3 K 56Q mutation H 3 K 56 acetylation H 3 K 56R histone H 3 disassembly transcriptional induction chromatin disassembly H 3 K 56Ac 2016-05-17 21:14:18 Figure https://plos.figshare.com/articles/figure/Commercial_H3_K56Ac_antibodies_are_non_specific_in_flies_/3939069 <p>The top schematic explains the mosaic nature of the wild type histone expression, indicated by GFP staining. All cells express H3 K56R, but the black patches express no endogenous wild type histones. Below are shown wing imaginal discs from flies with clones of cells expressing only H3 K56R (indicated by white arrows in the top row) that are marked by lack of GFP expression. Staining with the indicated H3 K56Ac antibodies showed no difference between H3 K56R mutant clones or the adjacent wild type clones, indicating that the antibodies are non specific. Representative images are shown.</p>