10.1371/journal.pone.0157700.g003 Han Chow Chua Han Chow Chua Emilie T. H. Christensen Emilie T. H. Christensen Kirsten Hoestgaard-Jensen Kirsten Hoestgaard-Jensen Leonny Y. Hartiadi Leonny Y. Hartiadi Iqbal Ramzan Iqbal Ramzan Anders A. Jensen Anders A. Jensen Nathan L. Absalom Nathan L. Absalom Mary Chebib Mary Chebib Kavain produced greater enhancement of GABA-elicited currents at α4β2δ than at α1β2γ2L GABA<sub>A</sub>Rs. Public Library of Science 2016 γ- Aminobutyric acid type decrease anaesthetic sensitivity binding assays kavain-mediated potentiation two-electrode voltage R subtypes 265M Anxiolytic Kava Extract modulatory effect α1β2γ2 L GABA Functional Characteristics Major Constituent anxiolytic kavalactone Xenopus oocytes Molecular Mechanism Extracts pepper plant benzodiazepine binding site Potentiates GABA subunit composition Piper methysticum 2016-06-22 19:56:56 Figure https://plos.figshare.com/articles/figure/Kavain_produced_greater_enhancement_of_GABA-elicited_currents_at_4_2_than_at_1_2_2L_GABA_sub_A_sub_Rs_/3929289 <p>(A) GABA concentration-response curves in the absence (black; <i>n</i> = 10) and presence (red; <i>n</i> = 6) of 300 μM kavain at α1β2γ2L GABA<sub>A</sub>Rs. The curve parameters are summarised in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157700#pone.0157700.t001" target="_blank">Table 1</a>. (B) GABA concentration-response curves in the absence (black; <i>n</i> = 4) and presence (red; <i>n</i> = 4) of 300 μM kavain at α4β2δ GABA<sub>A</sub>Rs. The curve parameters are summarised in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157700#pone.0157700.t001" target="_blank">Table 1</a>. (C) Superimposed current responses of maximal GABA in the absence (black) and presence (red) of 300 μM kavain at α1β2γ2L and α4β2δ GABA<sub>A</sub>Rs. (D) The effect of kavain on the maximal GABA current responses at α1β2γ2L (<i>n</i> = 6) and α4β2δ (<i>n</i> = 7) GABA<sub>A</sub>Rs was compared using the paired <i>t</i> test, and the significance levels are indicated with n.s. (not significant) and **** (<i>p</i> < 0.0001). Data are presented as mean ± SEM.</p>