Effects of fenretinide (4-HPR) on Wnt3a/β-catenin pathway. Barbara Bassani Desirèe Bartolini Arianna Pagani Elisa Principi Massimo Zollo Douglas M. Noonan Adriana Albini Antonino Bruno 10.1371/journal.pone.0154111.g005 https://plos.figshare.com/articles/figure/Effects_of_fenretinide_4-HPR_on_Wnt3a_-catenin_pathway_/3915096 <p>WB analysis for proteins involved in Wnt3a/β-catenin pathway show decreased level of Wnt3a in DAOY cells (A) treated for 24 hours with 4-HPR at the highest dose while Wnt3a down-regulation started at 2.5 μM in ONS-76 cells (B). Axin-1 and pGSK3β decreased in DAOY and ONS-76 cells treated as above (C-F). Decreased levels of β-catenin was observed at 6 and 24 hours following 4-HPR treatment in DAOY and ONS-76 cells (G, H).</p> 2016-07-01 17:51:46 PARP Medulloblastoma Cell Spheroids Medulloblastoma MB cell migration Western Blot analysis 4- HPR treatment 4- HPR targets well-tolerated cancer chemopreventive MB tumor cell growth cell cycle arrest ONS -76 cells 4- HPR induction all-trans retinoic acid Wnt 3a Pathway DAOY ONS -76 spheroid formation Sox -2 gene expression ROS oxygen reactive species cell cycle effects BTIC ABCG MB cell lines β- catenin PCR flow cytometry analysis surface markers CD 133 childhood brain malignancy ERK BITIC 4- HPR MB spheroids model 4- HPR ability