Effects of fenretinide (4-HPR) on Wnt3a/β-catenin pathway.
Barbara Bassani
Desirèe Bartolini
Arianna Pagani
Elisa Principi
Massimo Zollo
Douglas M. Noonan
Adriana Albini
Antonino Bruno
10.1371/journal.pone.0154111.g005
https://plos.figshare.com/articles/figure/Effects_of_fenretinide_4-HPR_on_Wnt3a_-catenin_pathway_/3915096
<p>WB analysis for proteins involved in Wnt3a/β-catenin pathway show decreased level of Wnt3a in DAOY cells (A) treated for 24 hours with 4-HPR at the highest dose while Wnt3a down-regulation started at 2.5 μM in ONS-76 cells (B). Axin-1 and pGSK3β decreased in DAOY and ONS-76 cells treated as above (C-F). Decreased levels of β-catenin was observed at 6 and 24 hours following 4-HPR treatment in DAOY and ONS-76 cells (G, H).</p>
2016-07-01 17:51:46
PARP
Medulloblastoma Cell Spheroids Medulloblastoma
MB cell migration
Western Blot analysis
4- HPR treatment
4- HPR targets
well-tolerated cancer chemopreventive
MB tumor cell growth
cell cycle arrest
ONS -76 cells
4- HPR induction
all-trans retinoic acid
Wnt 3a Pathway
DAOY
ONS -76 spheroid formation
Sox -2 gene expression
ROS
oxygen reactive species
cell cycle effects
BTIC
ABCG
MB cell lines
β- catenin
PCR
flow cytometry analysis
surface markers CD 133
childhood brain malignancy
ERK
BITIC
4- HPR
MB spheroids model
4- HPR ability