Survivin-ClpXP interaction. Jae Ho Seo Dayana B. Rivadeneira M. Cecilia Caino Young Chan Chae David W. Speicher Hsin-Yao Tang Valentina Vaira Silvano Bosari Alessandro Palleschi Paolo Rampini Andrew V. Kossenkov Lucia R. Languino Dario C. Altieri 10.1371/journal.pbio.1002507.g001 https://plos.figshare.com/articles/figure/Survivin-ClpXP_interaction_/3908997 <p>(<b>A</b>) Heatmap of proteomics identification of mitochondrial proteins co-precipitated with an antibody to survivin (SVV) or control IgG from PC3 mitochondrial extracts. Two independent replicates per condition are shown. A fold enrichment in spectra counts (SVV/IgG) is shown. (<b>B–D</b>) Mitochondrial extracts from PC3 cells were immunoprecipitated (IP) with antibodies to survivin (SVV) (<b>B</b>), ClpP (<b>C</b>), ClpP or ClpX (<b>D</b>), or non-binding IgG and analyzed by western blotting. TCE, total cell extracts. MTE, mitochondrial extracts. CS, citrate synthase. (<b>E</b>) Submitochondrial fractions representative of outer membrane (OM), inter-membrane space (IMS), inner membrane (IM), or matrix were isolated from PC3 cells and analyzed by western blotting. (<b>F</b>) PC3 cells were treated with vehicle (Veh) or small molecule survivin (SVV) suppressant YM155 and analyzed by western blotting. (<b>G</b>) PC3 cells in normoxia (N) or hypoxia (H) were transfected with control non-targeting siRNA (Ctrl) or survivin (SVV)-directed siRNA and analyzed by western blotting. (<b>H</b>) MCF-7 cells were transfected with vector or mitochondrial-targeted (mt) survivin (SVV) cDNA and analyzed by western blotting. Expo, exposure.</p> 2016-07-07 16:57:45 mitochondrial bioenergetics oncoprotein survivin ClpXP associates Deregulated mitochondrial respiration tumor cell proliferation patient survival misfolded SDHB Metastasis Mitochondria cell motility AMPK ATP production proteotoxic stress causes oxidative stress Mitochondrial Unfoldase-Peptidase Complex ClpXP Controls Bioenergetics Stress metastatic competence tumor cells ClpXP subunits ClpP ClpXP-directed proteostasis chain Complex II subunit succinate dehydrogenase B abolishes metastatic dissemination proteomics screen impairing oxidative phosphorylation mitochondrial unfoldase-peptidase buffer oxidative stress