Michaelis−Menten kinetics of dansylcadaverine and <i>N</i>,<i>N</i>-dimethylcasein conjugation by human FXIIIa in the presence of NSGM 13. Al-HoraniRami A. KaruturiRajesh LeeMichael AfosahDaniel K. DesaiUmesh R. 2016 <p>The initial rate of conjugation at (A) various dansylcadaverine concentrations (0–750 μM) and fixed dimethylcasein concentration (5 mg/mL) or (B) various dimethylcasein concentrations (0–5 mg/mL) and fixed dansylcadaverine concentration (250 μM) was measured spectrofluorometrically in pH 8.0 buffer at 37°C. Solid lines represent nonlinear regressional fits to the data by the standard Michaelis− Menten <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0160189#pone.0160189.e004" target="_blank">Eq 4</a> to yield <i>K</i><sub><i>M</i></sub> and <i>V</i><sub><i>MAX</i></sub>. See details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0160189#sec011" target="_blank">Materials and Methods</a>.</p>