%0 Figure %A Al-Horani, Rami A. %A Karuturi, Rajesh %A Lee, Michael %A Afosah, Daniel K. %A Desai, Umesh R. %D 2016 %T Michaelis−Menten kinetics of dansylcadaverine and N,N-dimethylcasein conjugation by human FXIIIa in the presence of NSGM 13. %U https://plos.figshare.com/articles/figure/Michaelis_Menten_kinetics_of_dansylcadaverine_and_i_N_i_i_N_i_-dimethylcasein_conjugation_by_human_FXIIIa_in_the_presence_of_NSGM_13_/3877935 %R 10.1371/journal.pone.0160189.g004 %2 https://plos.figshare.com/ndownloader/files/6079086 %K NSGM %K allosteric process %K Fluorescence studies %K 22 variably sulfated GAGs %K non-saccharide GAG mimetics %K orthosteric inhibition %K novel FXIIIa inhibitors %K cysteine protease papain %K allosteric FXIIIa inhibitors %K allosteric mechanism %K sulfated non-saccharide agents %K Non-Saccharide Glycosaminoglycan Mimetics %K Allosteric Inhibition %K Exhibit Promising Inhibition Profile Factor XIIIa %K acid residues %K FXIIIa-mediated polymerization %K sulfated glycosaminoglycans %K Factor XIIIa %K binding affinity %K inhibition potency %K allosteric inhibition %K GAG-binding proteins factor Xa %K undecasulfated quercetin analog %K design FXIIIa inhibitors %K coagulation process %X

The initial rate of conjugation at (A) various dansylcadaverine concentrations (0–750 μM) and fixed dimethylcasein concentration (5 mg/mL) or (B) various dimethylcasein concentrations (0–5 mg/mL) and fixed dansylcadaverine concentration (250 μM) was measured spectrofluorometrically in pH 8.0 buffer at 37°C. Solid lines represent nonlinear regressional fits to the data by the standard Michaelis− Menten Eq 4 to yield KM and VMAX. See details in Materials and Methods.

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