Frequency of CD4<sup>+</sup> TGFβRII<sup>+</sup> T cells in tissues of tolerized C57BL/6 mice after single challenge with OVA plus CT. Rebekah S. Gilbert Ryoki Kobayashi Shinichi Sekine Kohtaro Fujihashi 10.1371/journal.pone.0027501.g003 https://plos.figshare.com/articles/figure/_Frequency_of_CD4_TGF_946_RII_T_cells_in_tissues_of_tolerized_C57BL_6_mice_after_single_challenge_with_OVA_plus_CT_/386467 <p><i>A</i>. Mononuclear cells were isolated from PPs, MLNs, iLP and spleens of C57BL/6 mice 7 days after 30 mg of OVA feeding (0) and at 0.5, 2 and 4 h after single challenge with 1 mg of OVA plus 10 µg of CT (OVA plus CT). Cells were then stained with FITC-conjugated anti-hTGFβRII, APC-labeled anti-CD4, and biotin-tagged anti-CD3 mAbs followed by PerCP-Cy™5.5-conjugated streptavidin. <i>B.</i> Cells were isolated as described above and stained with FITC-conjugated anti-hTGFβRII, APC-labeled anti-CD4, PE-labeled anti-hFoxp3, and biotin-tagged anti-CD3 mAbs followed by PerCP-Cy™5.5-conjugated streptavidin. Samples were subjected to flow cytometry analysis by FACSCalibur®. <i>n</i> = 5 mice per time point, *<i>p</i><0.05 compared to sham-tolerized and OVA plus CT challenged controls.</p> 2011-11-07 01:47:47 cells tissues tolerized mice ova