10.1371/journal.pone.0163320.g001 Reenu Kumari Reenu Kumari Surender Kumar Surender Kumar Lakhmir Singh Lakhmir Singh Vipin Hallan Vipin Hallan Construct preparation for yeast two hybrid. Public Library of Science 2016 WT 7 days post inoculation yeast two-hybrid screen Nicotiana benthamiana plants movement dpi Apoplastic Ascorbate Oxidase Plant CMV Cucumber mosaic virus TRV inoculated control plants MP Tobacco rattle virus vector CsAO 4 overexpressing Arabidopsis thaliana infection Cucumber Mosaic Virus Associates virus accumulation protein cell wall periphery 2016-09-26 17:30:02 Figure https://plos.figshare.com/articles/figure/Construct_preparation_for_yeast_two_hybrid_/3861639 <p>A) Diagram of CMV MP showing cysteine and histidine (Cys-His) rich region (126–194 aa); and RNA binding regions (174–233 aa). N-terminal region (MPn: 1–180 aa); C-terminal region (MPc: 101–279 aa) of MP; and complete MP (1–279 aa) used to prepare constructs in the pGBKT7 vector for yeast two-hybrid screening. B) Diagram of CsAO4 describing the location of the signal peptide, and of domains selected from three regions: Full AO (FAO: 1-578aa); N-terminal AO region (AO-N: 44-161aa); C-terminal AO region (AO-C: 170-336aa); middle AO region (AO-M: 453-561aa); and the signal peptide (1–33 aa) highlighted in grey color.</p>