10.1371/journal.pone.0163752.g004 Francisco Javier Pavón Francisco Javier Pavón Eva María Marco Eva María Marco Mariam Vázquez Mariam Vázquez Laura Sánchez Laura Sánchez Patricia Rivera Patricia Rivera Ana Gavito Ana Gavito Virginia Mela Virginia Mela Francisco Alén Francisco Alén Juan Decara Juan Decara Juan Suárez Juan Suárez Elena Giné Elena Giné José Antonio López-Moreno José Antonio López-Moreno Julie Chowen Julie Chowen Fernando Rodríguez-de-Fonseca Fernando Rodríguez-de-Fonseca Antonia Serrano Antonia Serrano María Paz Viveros María Paz Viveros Relative mRNA expression of endocannabinoid signaling-related proteins in the spleen of rats exposed to alcohol during adolescence and control rats grouped by sex. Public Library of Science 2016 CB 2 expression 4- day drinking interleukin -6 concentrations N-acyl-phosphatidylethanolamine-specific phospholipase D expression area-specific expression patterns Young Adult Rats Intermittent alcohol exposure cannabinoid receptor CB 1 ECS adult rats splenic endocannabinoid signaling-related proteins Endocannabinoid Signaling-Related Proteins alcohol-exposed rats C-X 3-C motif PPAR DID plasma pro-inflammatory cytokines Adolescent Intermittent Alcohol Exposure alcohol consumption TNF 2016-09-23 17:33:52 Figure https://plos.figshare.com/articles/figure/Relative_mRNA_expression_of_endocannabinoid_signaling-related_proteins_in_the_spleen_of_rats_exposed_to_alcohol_during_adolescence_and_control_rats_grouped_by_sex_/3857091 <p>(A) CB<sub>1</sub> mRNA levels (fold change); (B) CB<sub>2</sub> mRNA levels (fold change); (C) PPARα mRNA levels (fold change); (D) NAPE-PLD mRNA levels (fold change); (E) DGLα mRNA levels (fold change); (F) DGLβ mRNA levels (fold change); (G) FAAH mRNA levels (fold change); (H) MGL mRNA levels (fold change); and (I) FAT/CD36 mRNA levels (fold change). The bars represent means ± SEM. The data were analyzed by two-way ANOVA and significant main effects or interaction between factors was indicated as follows: (*) p<0.05 and (**) p<0.01 denote significant main effect of <i>adolescent alcohol exposure</i>; (<sup>###</sup>) p<0.001 denotes significant interaction between <i>adolescent alcohol exposure</i> and <i>sex</i>. (<sup>aaa</sup>) p<0.001 denotes significant differences compared to female control rats after <i>post hoc</i> tests; (<sup>bbb</sup>) p<0.001 denotes significant differences compared to female alcohol-exposed rats after <i>post hoc</i> tests.</p>