Weidong Du, William Yang, Burton B. L. Yang, Bing Deng, Zhaoqun Fang, Ling Wan Shan, Sze Jeyapalan, Zina Zhang, Yaou Seth, Arun J. Yee, Albert Versican G3 modulated breast cancer cell apoptosis induced by chemotherapeutic drugs through activation of EGFR related signaling. <p>a) 1×10<sup>4</sup> G3- and vector-transfected MT-1, MDA-MB-468, 66c14, 4T07, and 4T1 cells were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, cells were treated with 2 µM Docetaxel for 24 hours. Cell viability was tested by WST-1 assays. b) All cells were treated with 8 µM Doxorubicin, and subjected to WST-1 assays. c) All cells were treated with 10 µM Epirubicin, and subjected to WST-1 assays. Compared with vector control group, <i>n</i> = 6, * <i>p</i><0.05, **<i>p</i><0.01, analyzed with <i>t</i>-test. d) Treated with 40 µM C2-ceramide, 2 µM Docetaxel, 8 µM Doxorubicin, 10 µM Epirubicin, 15 mM Cyclophosphamide, or 30 µM Trastuzumab for 6 hours, G3-expressing and vector- expressing 66c14 cells were processed to lysates and subjected to immunoblotting with antibodies to pSAPK/JNK, SAPK/JNK, ERK2, pERK, and β-actin. e) G3-transfected and vector-transfected 66c14 cells (1×10<sup>4</sup>) were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, we added Docetaxel (2 µM), and EGF (20 ng/ml), AG 1478 (2.0 µM), PD 98059 (50 µM), or SP 600125 (100 nM) cultured for 24 hours. WST-1 Cell Survival Assays were used to analyze cell viability. f) G3-transfected and vector-transfected 66c14 cells (1×10<sup>4</sup>) were also treated with Doxorubicin (8 µM), and EGF (20 ng/ml), AG 1478 (2.0 µM), PD 98059 (50 µM), or SP 600125 (100 nM) cultured for 24 hours. Cell viability was analyzed by WST-1 assays. Compared with vector control group, <i>n</i> = 8, * <i>p</i><0.05, **<i>p</i><0.01, analyzed with <i>t</i>-test.</p> g3;modulated;cancer;apoptosis;induced;chemotherapeutic;drugs;activation;egfr 2011-11-09
    https://plos.figshare.com/articles/figure/_Versican_G3_modulated_breast_cancer_cell_apoptosis_induced_by_chemotherapeutic_drugs_through_activation_of_EGFR_related_signaling_/385469
10.1371/journal.pone.0026396.g006