10.1371/journal.pone.0026396.g006
William Weidong Du
William
Weidong Du
Burton B. Yang
Burton B.
Yang
Bing L. Yang
Bing
L. Yang
Zhaoqun Deng
Zhaoqun
Deng
Ling Fang
Ling
Fang
Sze Wan Shan
Sze
Wan Shan
Zina Jeyapalan
Zina
Jeyapalan
Yaou Zhang
Yaou
Zhang
Arun Seth
Arun
Seth
Albert J. Yee
Albert
J. Yee
Versican G3 modulated breast cancer cell apoptosis induced by chemotherapeutic drugs through activation of EGFR related signaling.
Public Library of Science
2011
g3
modulated
cancer
apoptosis
induced
chemotherapeutic
drugs
activation
egfr
2011-11-09 01:31:09
Figure
https://plos.figshare.com/articles/figure/_Versican_G3_modulated_breast_cancer_cell_apoptosis_induced_by_chemotherapeutic_drugs_through_activation_of_EGFR_related_signaling_/385469
<p>a) 1×10<sup>4</sup> G3- and vector-transfected MT-1, MDA-MB-468, 66c14, 4T07, and 4T1 cells were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, cells were treated with 2 µM Docetaxel for 24 hours. Cell viability was tested by WST-1 assays. b) All cells were treated with 8 µM Doxorubicin, and subjected to WST-1 assays. c) All cells were treated with 10 µM Epirubicin, and subjected to WST-1 assays. Compared with vector control group, <i>n</i> = 6, * <i>p</i><0.05, **<i>p</i><0.01, analyzed with <i>t</i>-test. d) Treated with 40 µM C2-ceramide, 2 µM Docetaxel, 8 µM Doxorubicin, 10 µM Epirubicin, 15 mM Cyclophosphamide, or 30 µM Trastuzumab for 6 hours, G3-expressing and vector- expressing 66c14 cells were processed to lysates and subjected to immunoblotting with antibodies to pSAPK/JNK, SAPK/JNK, ERK2, pERK, and β-actin. e) G3-transfected and vector-transfected 66c14 cells (1×10<sup>4</sup>) were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, we added Docetaxel (2 µM), and EGF (20 ng/ml), AG 1478 (2.0 µM), PD 98059 (50 µM), or SP 600125 (100 nM) cultured for 24 hours. WST-1 Cell Survival Assays were used to analyze cell viability. f) G3-transfected and vector-transfected 66c14 cells (1×10<sup>4</sup>) were also treated with Doxorubicin (8 µM), and EGF (20 ng/ml), AG 1478 (2.0 µM), PD 98059 (50 µM), or SP 600125 (100 nM) cultured for 24 hours. Cell viability was analyzed by WST-1 assays. Compared with vector control group, <i>n</i> = 8, * <i>p</i><0.05, **<i>p</i><0.01, analyzed with <i>t</i>-test.</p>