10.1371/journal.pone.0026396.g006 William Weidong Du William Weidong Du Burton B. Yang Burton B. Yang Bing L. Yang Bing L. Yang Zhaoqun Deng Zhaoqun Deng Ling Fang Ling Fang Sze Wan Shan Sze Wan Shan Zina Jeyapalan Zina Jeyapalan Yaou Zhang Yaou Zhang Arun Seth Arun Seth Albert J. Yee Albert J. Yee Versican G3 modulated breast cancer cell apoptosis induced by chemotherapeutic drugs through activation of EGFR related signaling. Public Library of Science 2011 g3 modulated cancer apoptosis induced chemotherapeutic drugs activation egfr 2011-11-09 01:31:09 Figure https://plos.figshare.com/articles/figure/_Versican_G3_modulated_breast_cancer_cell_apoptosis_induced_by_chemotherapeutic_drugs_through_activation_of_EGFR_related_signaling_/385469 <p>a) 1×10<sup>4</sup> G3- and vector-transfected MT-1, MDA-MB-468, 66c14, 4T07, and 4T1 cells were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, cells were treated with 2 µM Docetaxel for 24 hours. Cell viability was tested by WST-1 assays. b) All cells were treated with 8 µM Doxorubicin, and subjected to WST-1 assays. c) All cells were treated with 10 µM Epirubicin, and subjected to WST-1 assays. Compared with vector control group, <i>n</i> = 6, * <i>p</i><0.05, **<i>p</i><0.01, analyzed with <i>t</i>-test. d) Treated with 40 µM C2-ceramide, 2 µM Docetaxel, 8 µM Doxorubicin, 10 µM Epirubicin, 15 mM Cyclophosphamide, or 30 µM Trastuzumab for 6 hours, G3-expressing and vector- expressing 66c14 cells were processed to lysates and subjected to immunoblotting with antibodies to pSAPK/JNK, SAPK/JNK, ERK2, pERK, and β-actin. e) G3-transfected and vector-transfected 66c14 cells (1×10<sup>4</sup>) were inoculated and cultured in 10% FBS/DMEM medium in 96 well culture dishes for 12 hours. After cell attachment, we added Docetaxel (2 µM), and EGF (20 ng/ml), AG 1478 (2.0 µM), PD 98059 (50 µM), or SP 600125 (100 nM) cultured for 24 hours. WST-1 Cell Survival Assays were used to analyze cell viability. f) G3-transfected and vector-transfected 66c14 cells (1×10<sup>4</sup>) were also treated with Doxorubicin (8 µM), and EGF (20 ng/ml), AG 1478 (2.0 µM), PD 98059 (50 µM), or SP 600125 (100 nM) cultured for 24 hours. Cell viability was analyzed by WST-1 assays. Compared with vector control group, <i>n</i> = 8, * <i>p</i><0.05, **<i>p</i><0.01, analyzed with <i>t</i>-test.</p>