%0 Figure %A Yokoyama, Tomohisa %A Tam, Justina %A Kuroda, Shinji %A W. Scott, Ailing %A Aaron, Jesse %A Larson, Tim %A Shanker, Manish %A M. Correa, Arlene %A Kondo, Seiji %A Roth, Jack A. %A Sokolov, Konstantin %A Ramesh, Rajagopal %D 2011 %T Analysis for molecular markers of autophagy and apoptosis in C225-NP-treated cancer cells. %U https://plos.figshare.com/articles/figure/_Analysis_for_molecular_markers_of_autophagy_and_apoptosis_in_C225_NP_treated_cancer_cells_/385459 %R 10.1371/journal.pone.0025507.g005 %2 https://plos.figshare.com/ndownloader/files/715115 %K molecular %K markers %K autophagy %K apoptosis %K c225-np-treated %K cancer %X

(a) Cellular proteins were lysed at the indicated time after treatment with IgG-NP particles or C225-NP (0.6×1010 particles) and subjected to Western blotting. In HCC827 but not in H520 cells, increased PARP cleavage and LC3-II was observed at 48 h and 72 h after treatment with C225-NP and when compared with IgG-NP treatment and untreated control. The intensities of the amount of LC3-II bands were semi-quantified by ImageJ software (National Institutes of Health, Bethesda, MD) (b) HCC827 cells were treated with IgG-NP or C225-NP for 68 hrs, cells were further cultured with or without protease inhibitors [10 µg/ml E-64-d and 10 µg/ml pepstatin A (BIOMOL International L.P., Plymouth Meeting, PA)] for 4 hrs. Cellular proteins were lysed and immunoblotted with anti-LC3 antibody. LC3-II protein levels were increased in the presence of protease inhibitors in all of the groups indicating occurrence of autophagy. The intensities of the amount of LC3-II bands were semi-quantified by ImageJ software (National Institutes of Health).

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