%0 Figure %A Tharad, Megha %A Kumar Samuchiwal, Sachin %A Bhalla, Kuhulika %A Ghosh, Anamika %A Kumar, Krishan %A Kumar, Sushil %A Ranganathan, Anand %D 2011 %T Representation of ESAT6 : HCL1 protein-protein disruption by CFP10 in bacterial three-hybrid system. %U https://plos.figshare.com/articles/figure/_Representation_of_ESAT6_HCL1_protein_protein_disruption_by_CFP10_in_bacterial_three_hybrid_system_/385455 %R 10.1371/journal.pone.0027503.g004 %2 https://plos.figshare.com/ndownloader/files/715106 %K esat6 %K hcl1 %K protein-protein %K disruption %K cfp10 %K bacterial %K three-hybrid %X

(A) X-Gal indicator plate with and without arabinose patched with ESAT6pBTnn + HCL1pTRGnn + pMTSA; and ESAT6pBTnn + HCL1pTRGnn + CFP10pMTSA. Blue colony turns white when CFP10 is allowed to express in the presence of 1% arabinose. (B) Time course liquid β-galactosidase assay: β-galactosidase activity of above triple co-transformants: (▪) ESAT6pBTnn + HCL1pTRGnn + pMTSA; and (•) ESAT6pBTnn + HCL1pTRGnn + CFP10pMTSA is plotted against time-points of bacterial culture growth with 0 time-point being the point of arabinose induction. Standard deviation of the activities obtained in three separate assays is shown by error bars. (P<0.05 at all time-points beyond 60 minutes) (C) Arabinose gradient liquid β-galactosidase assay: Relative β-galactosidase activity of triple co-transformants (•) ESAT6pBTnn + HCL1pTRGnn + CFP10pMTSA; and (▪) ESAT6pBTnn + HCL1pTRGnn + pMTSA is plotted against arabinose concentration. The graph is the average of three separate assays and standard deviation is represented as error bars. (P<0.05 at all arabinose concentrations beyond 0.01%).

%I PLOS ONE