10.1371/journal.pbio.1001199.g004
Christopher A. Maxwell
Christopher
A. Maxwell
Javier Benítez
Javier
Benítez
Laia Gómez-Baldó
Laia
Gómez-Baldó
Ana Osorio
Ana
Osorio
Núria Bonifaci
Núria
Bonifaci
Ricardo Fernández-Ramires
Ricardo
Fernández-Ramires
Sylvain V. Costes
Sylvain
V. Costes
Elisabet Guinó
Elisabet
Guinó
Helen Chen
Helen
Chen
Gareth J. R. Evans
Gareth
J. R. Evans
Pooja Mohan
Pooja
Mohan
Isabel Català
Isabel
Català
Anna Petit
Anna
Petit
Helena Aguilar
Helena
Aguilar
Alberto Villanueva
Alberto
Villanueva
Alvaro Aytes
Alvaro
Aytes
Jordi Serra-Musach
Jordi
Serra-Musach
Gad Rennert
Gad
Rennert
Flavio Lejbkowicz
Flavio
Lejbkowicz
Paolo Peterlongo
Paolo
Peterlongo
Siranoush Manoukian
Siranoush
Manoukian
Bernard Peissel
Bernard
Peissel
Carla B. Ripamonti
Carla
B. Ripamonti
Bernardo Bonanni
Bernardo
Bonanni
Alessandra Viel
Alessandra
Viel
Anna Allavena
Anna
Allavena
Loris Bernard
Loris
Bernard
Paolo Radice
Paolo
Radice
Eitan Friedman
Eitan
Friedman
Bella Kaufman
Bella
Kaufman
Yael Laitman
Yael
Laitman
Maya Dubrovsky
Maya
Dubrovsky
Roni Milgrom
Roni
Milgrom
Anna Jakubowska
Anna
Jakubowska
Cezary Cybulski
Cezary
Cybulski
Bohdan Gorski
Bohdan
Gorski
Katarzyna Jaworska
Katarzyna
Jaworska
Katarzyna Durda
Katarzyna
Durda
Grzegorz Sukiennicki
Grzegorz
Sukiennicki
Jan Lubiński
Jan
Lubiński
Yin Yao Shugart
Yin
Yao Shugart
Susan M. Domchek
Susan
M. Domchek
Richard Letrero
Richard
Letrero
Barbara L. Weber
Barbara
L. Weber
Frans B. L. Hogervorst
Frans
B. L. Hogervorst
Matti A. Rookus
Matti
A. Rookus
J. Margriet Collee
J.
Margriet Collee
Peter Devilee
Peter
Devilee
Marjolijn J. Ligtenberg
Marjolijn
J. Ligtenberg
Rob B. van der Luijt
Rob
B. van der Luijt
Cora M. Aalfs
Cora
M. Aalfs
Quinten Waisfisz
Quinten
Waisfisz
Juul Wijnen
Juul
Wijnen
Cornelis E. P. van Roozendaal
Cornelis
E. P. van Roozendaal
Douglas F. Easton
Douglas
F. Easton
Susan Peock
Susan
Peock
Margaret Cook
Margaret
Cook
Clare Oliver
Clare
Oliver
Debra Frost
Debra
Frost
Patricia Harrington
Patricia
Harrington
D. Gareth Evans
D.
Gareth Evans
Fiona Lalloo
Fiona
Lalloo
Rosalind Eeles
Rosalind
Eeles
Louise Izatt
Louise
Izatt
Carol Chu
Carol
Chu
Diana Eccles
Diana
Eccles
Fiona Douglas
Fiona
Douglas
Carole Brewer
Carole
Brewer
Heli Nevanlinna
Heli
Nevanlinna
Tuomas Heikkinen
Tuomas
Heikkinen
Fergus J. Couch
Fergus
J. Couch
Noralane M. Lindor
Noralane
M. Lindor
Xianshu Wang
Xianshu
Wang
Andrew K. Godwin
Andrew K.
Godwin
Maria A. Caligo
Maria
A. Caligo
Grazia Lombardi
Grazia
Lombardi
Niklas Loman
Niklas
Loman
Per Karlsson
Per
Karlsson
Hans Ehrencrona
Hans
Ehrencrona
Anna von Wachenfeldt
Anna
von Wachenfeldt
Rosa Bjork Barkardottir
Rosa
Bjork Barkardottir
Ute Hamann
Ute
Hamann
Muhammad U. Rashid
Muhammad
U. Rashid
Adriana Lasa
Adriana
Lasa
Trinidad Caldés
Trinidad
Caldés
Raquel Andrés
Raquel
Andrés
Michael Schmitt
Michael
Schmitt
Volker Assmann
Volker
Assmann
Kristen Stevens
Kristen
Stevens
Kenneth Offit
Kenneth
Offit
João Curado
João
Curado
Hagen Tilgner
Hagen
Tilgner
Roderic Guigó
Roderic
Guigó
Gemma Aiza
Gemma
Aiza
Joan Brunet
Joan
Brunet
Joan Castellsagué
Joan
Castellsagué
Griselda Martrat
Griselda
Martrat
Ander Urruticoechea
Ander
Urruticoechea
Ignacio Blanco
Ignacio
Blanco
Laima Tihomirova
Laima
Tihomirova
David E. Goldgar
David
E. Goldgar
Saundra Buys
Saundra
Buys
Esther M. John
Esther
M. John
Alexander Miron
Alexander
Miron
Melissa Southey
Melissa
Southey
Mary B. Daly
Mary
B. Daly
Rita K. Schmutzler
Rita
K. Schmutzler
Barbara Wappenschmidt
Barbara
Wappenschmidt
Alfons Meindl
Alfons
Meindl
Norbert Arnold
Norbert
Arnold
Helmut Deissler
Helmut
Deissler
Raymonda Varon-Mateeva
Raymonda
Varon-Mateeva
Christian Sutter
Christian
Sutter
Dieter Niederacher
Dieter
Niederacher
Evgeny Imyamitov
Evgeny
Imyamitov
Olga M. Sinilnikova
Olga
M. Sinilnikova
Dominique Stoppa-Lyonne
Dominique
Stoppa-Lyonne
Sylvie Mazoyer
Sylvie
Mazoyer
Carole Verny-Pierre
Carole
Verny-Pierre
Laurent Castera
Laurent
Castera
Antoine de Pauw
Antoine
de Pauw
Yves-Jean Bignon
Yves-Jean
Bignon
Nancy Uhrhammer
Nancy
Uhrhammer
Jean-Philippe Peyrat
Jean-Philippe
Peyrat
Philippe Vennin
Philippe
Vennin
Sandra Fert Ferrer
Sandra
Fert Ferrer
Marie-Agnès Collonge-Rame
Marie-Agnès
Collonge-Rame
Isabelle Mortemousque
Isabelle
Mortemousque
Amanda B. Spurdle
Amanda
B. Spurdle
Jonathan Beesley
Jonathan
Beesley
Xiaoqing Chen
Xiaoqing
Chen
Sue Healey
Sue
Healey
Mary Helen Barcellos-Hoff
Mary
Helen Barcellos-Hoff
Marc Vidal
Marc
Vidal
Stephen B. Gruber
Stephen
B. Gruber
Conxi Lázaro
Conxi
Lázaro
Gabriel Capellá
Gabriel
Capellá
Lesley McGuffog
Lesley
McGuffog
Katherine L. Nathanson
Katherine
L. Nathanson
Antonis C. Antoniou
Antonis
C. Antoniou
Georgia Chenevix-Trench
Georgia
Chenevix-Trench
Markus C. Fleisch
Markus
C. Fleisch
Víctor Moreno
Víctor
Moreno
Miguel Angel Pujana
Miguel
Angel Pujana
Genetic interactions influencing epithelial apicobasal polarization.
Public Library of Science
2011
interactions
influencing
epithelial
apicobasal
2011-11-15 01:14:06
Figure
https://plos.figshare.com/articles/figure/_Genetic_interactions_influencing_epithelial_apicobasal_polarization_/384446
<p>(A) shRNA-mediated depletion of centrosome components impairs polarization. Representative bright-field images are shown for results of untreated and control vector pLKO.1, shRNA-<i>AURKA</i>, shRNA-<i>BRCA1</i>, shRNA-<i>HMMR</i>, or shRNA-<i>TPX2</i> transduced cultures of MCF10A cells in rBM. Magnification is equivalent for all images and scale bars represent 20 µm. (B) Acini architecture was quantified from bright-field images of cultures treated as described above. For comparison between experiments, all values were normalized to untreated cultures within experiments and differences assessed statistically relative to pLKO.1. Shape factor values for single cells, or small clusters, are not plotted. The graph shows the results of at least four independent experiments. For all graphs, asterisks and circles indicate significant differences (two-sided <i>t</i> test <i>p</i><0.05 and <i>p</i><0.005, respectively) from controls (pLKO.1). (C) Representative bright-field images of acini from concurrent depletions (shRNA-mediated) as indicated. (D) <i>AURKA-HMMR</i> interact in the regulation of polarization: <i>HMMR</i> depletion rescues the abnormality seen in the shRNA-<i>AURKA</i> assay. Graph shows the results of three independent experiments. (E) Quantification of acini per well confirms the genetic interaction between <i>AURKA</i> and <i>HMMR</i>. Graph shows the results of duplicate experiments. (F) <i>TPX2</i> depletion is suppressive to abnormalities caused by shRNA-<i>BRCA1</i> and shRNA-<i>HMMR</i>. Graph shows the results of at least three independent experiments. (G) Prior to the shRNA assays, published data proposed the hypothesis of a signaling pathway from TPX2 to RHAMM regulating polarization; degradation of the microtubule-associated factor RHAMM, through BRCA1, was predicted as key to polarization. However, several observations from the single and concurrent depletion assays (depleted proteins are indicated in grey font) diverged from the expected results (divergent observations are italicized). RHAMM depletion impaired polarization in a manner that was rescued by concurrent depletion of AURKA or TPX2, but not BRCA1. On the other hand, concurrent depletion of BRCA1 and TPX2 revealed normal acini.</p>