Examination of putative genes, all encoded on pOLA52, pIS15_43 and pIS04_68 that might influence the biofilm or motility behavior of the bacterial host. MadsenJonas Stenløkke RiberLeise KotWitold BasfeldAlrun BurmølleMette Hestbjerg HansenLars Johannes SørensenSøren 2016 <p><i>Bottom</i>: CV biofilm assay. <i>E</i>. <i>coli</i> harboring constructs with the <i>mrkABCDF</i> cassette (<i>mrk</i>) formed more biofilm than those without but no difference was found among the biofilm formers (Error bars denote ±SEM, n = 3). <i>Middle</i>: swimming motility assay. Pictures were taken after 8h or 16h at 37°C. No differences were found (all swam equal distances, ++) with the exception of the negative control MG1655 <i>ΔflhD</i> (did not swim, −). MG1655 was used a positive control for swimming (not shown). <i>Top</i>: CR assay. Pictures were taken after 2 days at 30°C. No differences were found (equal amounts of CR was bound, ++) with the exception of pRham-P<sub><i>rham</i></sub><i>xeal</i> for which less CR was bound by the colony (+). <i>Pseudomonas aeruginosa</i> PA14 and <i>P</i>. <i>aeruginosa</i> PA14 <i>Δpel</i> were used as positive and negative controls, respectively (not shown).</p>