%0 Figure %A Madsen, Jonas Stenløkke %A Riber, Leise %A Kot, Witold %A Basfeld, Alrun %A Burmølle, Mette %A Hestbjerg Hansen, Lars %A Johannes Sørensen, Søren %D 2016 %T Examination of putative genes, all encoded on pOLA52, pIS15_43 and pIS04_68 that might influence the biofilm or motility behavior of the bacterial host. %U https://plos.figshare.com/articles/figure/Examination_of_putative_genes_all_encoded_on_pOLA52_pIS15_43_and_pIS04_68_that_might_influence_the_biofilm_or_motility_behavior_of_the_bacterial_host_/3831615 %R 10.1371/journal.pone.0162390.g007 %2 https://plos.figshare.com/ndownloader/files/5978145 %K planktonic behavior %K type 3 fimbriae %K chromosomal elements %K study differences %K fimbriae operon %K Klebsiella pneumoniae chromosome %K Unique Promoter %K Affecting Host Motility %K sessile behavior %K Enhances Conjugal Plasmid Transfer Horizontal gene transfer %K HGT %K Klebsiella promoter %K mrkABCDF %K Type 3 Fimbriae Encoded %K conjugative plasmids %K transcriptional activator %K type plasmids %K Exceptional Phenotype %K expression %X

Bottom: CV biofilm assay. E. coli harboring constructs with the mrkABCDF cassette (mrk) formed more biofilm than those without but no difference was found among the biofilm formers (Error bars denote ±SEM, n = 3). Middle: swimming motility assay. Pictures were taken after 8h or 16h at 37°C. No differences were found (all swam equal distances, ++) with the exception of the negative control MG1655 ΔflhD (did not swim, −). MG1655 was used a positive control for swimming (not shown). Top: CR assay. Pictures were taken after 2 days at 30°C. No differences were found (equal amounts of CR was bound, ++) with the exception of pRham-Prhamxeal for which less CR was bound by the colony (+). Pseudomonas aeruginosa PA14 and P. aeruginosa PA14 Δpel were used as positive and negative controls, respectively (not shown).

%I PLOS ONE