RT-PCR analysis results are affected by sampling system, RNA extraction method and the choice of reference gene. Anke Meyer Federico Paroni Kathrin Günther Gitanjali Dharmadhikari Wolfgang Ahrens Sørge Kelm Kathrin Maedler 10.1371/journal.pone.0161778.g003 https://plos.figshare.com/articles/figure/RT-PCR_analysis_results_are_affected_by_sampling_system_RNA_extraction_method_and_the_choice_of_reference_gene_/3797154 <p><i>SIGLEC-7</i> gene expression profiles of Series B with additional DNAse digestion. Blood was collected from 12 donors; triplicates from each donor were reverse transcribed and experimental duplicates from each sample run in Real-Time PCR reactions. Data were normalized to <b>(A)</b> <i>PPIA</i>, <b>(B)</b> <i>β-ACTIN</i>, <b>(C)</b> <i>β-TUBULIN</i> and (<b>D</b>) to a combination of these 3 reference genes. Results are shown as relative to the randomly chosen subject #1. A-C show means of experimental duplicates ± SE, D shows means of pooled triplicates from each donor.</p> 2016-08-30 17:33:51 gene expression results Norgen RNA extraction methods Pre-analytix PAXgene TM RNA extraction system TUBULIN PAX RNA extraction Kits PAXgene TM blood collection system Tempus TM collection tubes RNA concentration ACTIN PAX TM collection system PAXgene TM collection system blood samples Human Blood mRNA Isolation Tempus TM collection Tempus TM collection systems RNA isolation methods RNA isolation system PPIA SIGLEC -7. Results Real-Time PCR analyses gene expression analyses PAXgene TM RNA extraction system PAXgene TM blood collection method