RT-PCR analysis results are affected by sampling system, RNA extraction method and the choice of reference gene.
Anke Meyer
Federico Paroni
Kathrin Günther
Gitanjali Dharmadhikari
Wolfgang Ahrens
Sørge Kelm
Kathrin Maedler
10.1371/journal.pone.0161778.g003
https://plos.figshare.com/articles/figure/RT-PCR_analysis_results_are_affected_by_sampling_system_RNA_extraction_method_and_the_choice_of_reference_gene_/3797154
<p><i>SIGLEC-7</i> gene expression profiles of Series B with additional DNAse digestion. Blood was collected from 12 donors; triplicates from each donor were reverse transcribed and experimental duplicates from each sample run in Real-Time PCR reactions. Data were normalized to <b>(A)</b> <i>PPIA</i>, <b>(B)</b> <i>β-ACTIN</i>, <b>(C)</b> <i>β-TUBULIN</i> and (<b>D</b>) to a combination of these 3 reference genes. Results are shown as relative to the randomly chosen subject #1. A-C show means of experimental duplicates ± SE, D shows means of pooled triplicates from each donor.</p>
2016-08-30 17:33:51
gene expression results
Norgen RNA extraction methods
Pre-analytix PAXgene TM RNA extraction system
TUBULIN
PAX RNA extraction Kits
PAXgene TM blood collection system
Tempus TM collection tubes
RNA concentration
ACTIN
PAX TM collection system
PAXgene TM collection system
blood samples
Human Blood mRNA Isolation
Tempus TM collection
Tempus TM collection systems
RNA isolation methods
RNA isolation system
PPIA
SIGLEC -7. Results
Real-Time PCR analyses
gene expression analyses
PAXgene TM RNA extraction system
PAXgene TM blood collection method