10.1371/journal.pone.0028491.g002 Weidong Han Weidong Han Jie Sun Jie Sun Lifeng Feng Lifeng Feng KaiFeng Wang KaiFeng Wang Da Li Da Li Qin Pan Qin Pan Yan Chen Yan Chen Wei Jin Wei Jin Xian Wang Xian Wang Hongming Pan Hongming Pan Hongchuan Jin Hongchuan Jin DNR induced autophagy in K562 cells. Public Library of Science 2011 induced autophagy k562 2011-12-02 02:18:18 Figure https://plos.figshare.com/articles/figure/_DNR_induced_autophagy_in_K562_cells_/378298 <p>A. K562 cells were incubated with varying concentrations of DNR for 24 hours or incubated with 1.25 µg/ml DNR for appropriate intervals. The switch of LC3-I to LC3-II was detected by immunoblotting. B. Immunoblotting for Beclin-1 using lysates from K562 treated with 1.25 µg/ml DNR for appropriate intervals. C. MDC staining of K562 treated with DNR. Cells treated with 1.25 µg/ml DNR in the presence or absence of 5 µM CQ for 24 hours were stained with MDC. D. K562 cells were treated with 1.25 µg/ml DNR for 24 hours, and stained by indrect immunofluorescence as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028491#s2" target="_blank">Marterial & Methods</a>. The distribution of endogenous LC3 was monitored at confocal microscope.</p>