10.1371/journal.pntd.0001398.g003
Manuel Castro de Moura
Manuel
Castro de Moura
Francesc Miro
Francesc
Miro
Jung Min Han
Jung Min
Han
Sunghoon Kim
Sunghoon
Kim
Antonio Celada
Antonio
Celada
Lluís Ribas de Pouplana
Lluís
Ribas de Pouplana
EhKRS protease processing.
Public Library of Science
2011
protease
2011-11-29 02:18:08
Figure
https://plos.figshare.com/articles/figure/_EhKRS_protease_processing_/378288
<p>Immunoblots analysis with α-EELP antibody of recombinant EhKRS protein digestions using <i>Entamoeba</i> lysates (A) or human leukocyte elastase (B). (A) 1 µg recombinant EhKRS was incubated at 37°C for 30 minutes without <i>E. histolytica</i> crude extract (Eh CE; lane 1) or with 0.5 µg Eh CE (lane 2–4); 1 µg Eh CE (lane 5–7); 5 µg Eh CE (lane 8–10) or 5 µg Eh CE plus protease inhibitors (lane 11–13) for 5, 15 or 30 minutes. (B) Digestion of 1 µg recombinant EhKRS protein with elastase for 30 minutes at 37°C. (C) EhMRS protease processing. Digestion of 1 µg recombinant EhMRS at 5, 15 and 30 minutes with 1 µg of Eh CE (lane 2–4) or 5 µg Eh CE (lane 5–7). Recombinant EhMRS control without Eh CE (lane 1). Digestion products were detected by immunoblot using α-EELP antibody. Arrow shows EELP product resulting from recombinant EhKRS or EhMRS. Asterisk denotes recombinant EELP with 6 His tag plus 36 amino acids at the N-terminal. Boxed sequence corresponds to the N-terminal sequence of EELP after digestion from EhKRS by elastase, as determined by Edman degradation.</p>