10.1371/journal.pone.0029099.g005
Josette
M. Douville
Josette
M.
Douville
David
Y. C. Cheung
David
Y.
C. Cheung
Krista
L. Herbert
Krista
L.
Herbert
Teri Moffatt
Teri
Moffatt
Jeffrey
T. Wigle
Jeffrey
T.
Wigle
Increased MEOX1 or MEOX2 expression leads to increased endothelial
cell senescence.
Public Library of Science
2011
meox1
meox2
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2011-12-20 00:21:09
Figure
https://plos.figshare.com/articles/figure/_Increased_MEOX1_or_MEOX2_expression_leads_to_increased_endothelial_cell_senescence_/371269
<p>A) Representative flow cytometry showing the density of BrdU<sup>+</sup>
endothelial cells (upper left and right quadrants). HUVECs were transduced
with N-terminal FLAG tagged MEOX1 and MEOX2 adenoviral constructs at a multiplicity
of infection (MOI) of 100; 48 hours later, cells were labeled with BrdU for
one hour prior to fixation. DNA was stained with 7-aminoactinomycin D (7-AAD).
B) Quantification of the flow cytometry data. Expression of MEOX1, MEOX2 and
MEOX2<sup>Q235E</sup> mutant decreased cellular proliferation comparable to
p53 (positive control) as assessed by BrdU incorporation into cycling cells.
C) Representative images showing SA-β-gal<sup>+</sup> cells (blue).
Nuclei were stained with hematoxylin (brown). D) Quantification of SA-β-gal<sup>+</sup>
cells shows that both MEOX1 and MEOX2 expression increased the number of senescent
HUVECs. In contrast, MEOX2<sup>Q235E</sup> expression did not alter the level
of endothelial cell senescence. HUVECs were transduced with N-terminal FLAG
tagged MEOX1 and MEOX2 adenoviral constructs at a MOI of 250; 48 hours later
cells were fixed and stained. D) MEOX proteins do not induced endothelial
cell apoptosis. HUVECs were transduced with FLAG tagged MEOX1, MEOX2 and MEOX2<sup>Q235E</sup>
adenoviral constructs at a MOI of 250; 48 hours later cells were fixed and
stained. Staurosporine was used as a positive control for apoptosis induction. *
Indicates a statistically significant change (p<0.05) when compared to
the EGFP control. ♦ Indicates a statistically significant difference
(p<0.05) between MEOX1 and MEOX2.</p>