Recommended acquisition parameters. L. WalkerSteven ArigaJunko R. MathiasJonathan CoothankandaswamyVeena XieXiayang DistelMartin W. KösterReinhard J. ParsonsMichael N. BhallaKapil T. SaxenaMeera S. MummJeff 2012 <p>*Promotes hands-free centering of subjects.</p><p>**Filling the well to eliminate the meniscus effect (∼340 µL) produced the best results, however, this practice proved impractical for HTS.</p>+<p>Promotes maximal throughput and consistently high Z'-factors (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029916#pone-0029916-t002" target="_blank">Table 2</a> & <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029916#pone-0029916-t003" target="_blank">3</a>).</p>#<p>Lethality issues were evident at the 400 hertz level when volume was ≤100 µL.</p>