Sequence and expression analysis of <i>Rubie</i>. Kristina A. Roberts Victoria E. Abraira Andrew F. Tucker Lisa V. Goodrich Nancy C. Andrews 10.1371/journal.pone.0029495.g003 https://plos.figshare.com/articles/figure/_Sequence_and_expression_analysis_of_Rubie_/363798 <p>(A) <i>Rubie</i> (EST BY232928) sequence displayed in genomic databases is derived from a partially-sequenced RIKEN cDNA clone and includes 388 bp, divided among three exons. Resequencing of the RIKEN clone revealed a full-length insert of 1404 bp, divided among five exons. (B) Expression of <i>Rubie</i> in embryonic (E15.5) and postnatal (P6) mouse inner ears was confirmed by RT-PCR. Expression was not observed in P6 hindbrain. The bottom PCR band represents use of an alternative splice site between exons 1 and 2 of <i>Rubie</i>. (C) <i>Rubie</i> expression was detected in the three sensory cristae (anterior crista, AC; lateral crista, LC; posterior crista, not shown) and the developing cochlea (Co) of sectioned E11.5 embryos by <i>in situ</i> hybridization. (D) No signal was detected using a <i>Rubie</i> sense control probe. <i>Rubie</i> (E) and <i>Bmp4</i> (F) exhibited similar expression patterns in the developing inner ear.</p> 2012-01-12 01:03:18 genetics and genomics developmental biology otolaryngology