DNA fragmentation induced in SCF spermatozoa is not detectable by TUNEL after fertilization.
Joanna E. Gawecka
Joel Marh
Michael Ortega
Yasuhiro Yamauchi
Monika A. Ward
W. Steven Ward
10.1371/journal.pone.0056385.g002
https://plos.figshare.com/articles/figure/_DNA_fragmentation_induced_in_SCF_spermatozoa_is_not_detectable_by_TUNEL_after_fertilization_/363277
<p>(a–d<sub>1</sub>) A Vas-SCF zygote stained with TUNEL and counterstained with DAPI 5 hrs after sperm injection, and visualized by confocal microscopy. (e–h<sub>1</sub>) A control zygote that was fixed and treated with DNAse I 10 mins after fertilization, and then stained by TUNEL. (i–l<sub>1</sub>) A control zygote that was treated with DNAse I 5 hrs after fertilization. Double columns in each panel present the same embryo at different focal planes. S – sperm, Mc – maternal chromatin, M – maternal pronucleus, P – paternal pronucleus, Pb – polar body. All images are shown at the same magnification (bars = 20 µm).</p>
2013-02-20 09:56:40
fragmentation
induced
scf
spermatozoa
detectable
tunel