The intergenic region of the <i>yscW-lcrF</i> operon is implicated in the temperature control of LcrF production. Katja Böhme Rebekka Steinmann Jens Kortmann Stephanie Seekircher Ann Kathrin Heroven Evelin Berger Fabio Pisano Tanja Thiermann Hans Wolf-Watz Franz Narberhaus Petra Dersch 10.1371/journal.ppat.1002518.g004 https://plos.figshare.com/articles/figure/_The_intergenic_region_of_the_yscW_lcrF_operon_is_implicated_in_the_temperature_control_of_LcrF_production_/352832 <p>(<b>A</b>) Schematic presentation of the reporter gene fusion harboring the <i>yscW-lcrF</i> intergenic region and different portions of <i>yscW</i> under control of the P<i><sub>BAD</sub></i> promoter. (<b>B</b>) <i>E. coli</i> K-12 harboring the different P<i><sub>BAD</sub></i>::<i>yscW-lcrF-lacZ</i> reporter plasmids (pED10, pED11 and pKB14) or the P<i><sub>BAD</sub></i>::<i>gnd-lacZ</i> control plasmid (pED05) were grown overnight in LB medium at 25°C or 37°C in the presence of 0.05% arabinose. β-Galactosidase activity from overnight cultures was determined and is given in mmol min<sup>−1</sup> mg<sup>−1</sup> for comparison. The data represent the average ± SD from at least three different experiments each done in duplicate. Data were analyzed by the Student's t test. Stars indicate the reporter activity that differed significantly between 25°C and 37°C with ** (P<0.01), and *** (P<0.001).</p> 2012-02-16 00:47:12 intergenic operon implicated lcrf