Matsuki, Tohru Zaka, Mariam Guerreiro, Rita P. van der Brug, Marcel Cooper, Jonathan A. Cookson, Mark R. A. Hardy, John W. Howell, Brian The Golgi extension and neuronal polarization phenotypes in <i>dab1</i> mutant neurons is dependent upon mouse strain background. <p>The appearance of the Golgi apparatus was examined in 100 µm thick sections of the hippocampus at birth by immunostaining for GRASP65 and Ctip2 to identify pyramidal neurons. <b>A</b> In wild-type animals the Golgi apparatus extends several microns into the presumptive apical dendrite in the hippocampi, but in C57BL/6 <i>dab1−/−</i> mutants it is convoluted near the nucleus. The Golgi appears more elongated in the Balb/c <i>dab1−/−</i> mutant mice. Nucleus to Golgi distances were measured on isolated cells (insets). Arrowheads represent points used for measurements. <b>B</b> The nucleus to Golgi tip distances are greater for wild-type than <i>dab1−/−</i> mutants, and greater for BALB/c versus C57BL/6 <i>dab1−/−</i> mutants (*,** p<0.0001, Student's t test). <b>C</b> The number of multiple axon bearing neurons in <i>dab1−/−</i> mutant neurons is reduced on the BALB/c background as compared to the C57BL/6 background. In both cases, knocking down Stk25 leads to a further reduction in neurons with multiple axons and the development of neurons with no axons (* p<0.001 compared to the respective EV-control samples, ** p = 0.01 compared between EV-control samples, Student's t test) (Bar = 20 µm) Values for C57BL/6 samples have been published previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031152#pone.0031152-Matsuki2" target="_blank">[34]</a> and are shown here for comparison to BALB/c samples.</p> golgi;neuronal;polarization;phenotypes;mutant;neurons 2013-02-20
    https://plos.figshare.com/articles/figure/_The_Golgi_extension_and_neuronal_polarization_phenotypes_in_dab1_mutant_neurons_is_dependent_upon_mouse_strain_background_/352803
10.1371/journal.pone.0031152.g005