10.1371/journal.ppat.1002536.g005 Flavia L. Ribeiro-Gomes Flavia L. Ribeiro-Gomes Nathan C. Peters Nathan C. Peters Alain Debrabant Alain Debrabant David L. Sacks David L. Sacks Dermal DCs infected with <i>L. major</i> display neutrophil markers. Public Library of Science 2012 dcs infected neutrophil 2012-02-16 00:45:23 Figure https://plos.figshare.com/articles/figure/_Dermal_DCs_infected_with_L_major_display_neutrophil_markers_/352723 <p>Mice were treated with control (GL113) or neutrophil-depleting (RB6-8C5 or 1A8) monoclonal antibodies 1 d before challenge in the ear dermis with 2×10<sup>5 </sup><i>Lm</i>-RFP metacyclic promastigotes. (A) Representative dot plots of CD11b<sup>+</sup>GR-1<sup>hi</sup>Ly6C<sup>int</sup> neutrophils (region 1) and CD11b<sup>+</sup>GR1<sup>int</sup>Ly6C<sup>hi</sup> inflammatory monocytes (region 2) in the GL113 or 1A8 treated mice. (B) Representative dot plots of CD11b<sup>+</sup>Ly6G<b><sup>+</sup></b>Ly6C<sup>int</sup> neutrophils (region 1) and CD11b<sup>+</sup>Ly6G<b><sup>−</sup></b>Ly6C<sup>hi</sup> inflammatory monocytes in the GL113 or RB6-8C5 depleted mice (region 2). (C) Mean total number per ear of neutrophils (PMN) or inflammatory monocytes (Mo) in neutrophil depleted or control treated, infected mice, +/− 1 s.d., 4–6 ears per group pooled from 2 independent experiments. (D–E) Mean total number of DCs (CD11c<sup>+</sup>MHCII<sup>+</sup>) per ear (D) and the mean percentage of RFP<b><sup>+</sup></b> DCs per ear (E) 1 d after infection, +/− 1 s.d., 8–10 ears per group, pooled from 3 independent experiments. (F) Representative dot plot of CD11c<b><sup>+</sup></b>MHCII<b><sup>+</sup></b>-gated RFP<b><sup>−</sup></b> uninfected and RFP<b><sup>+</sup></b> infected dermal DCs, 24 hr after infection. (G) Representative histogram plots of MPO stained, RFP<b><sup>−</sup></b> (gray filled) and RFP<b><sup>+</sup></b> (black line) DCs 24 hr after infection. (H) Mean percentage of RFP<b><sup>−</sup></b> or RFP<b><sup>+</sup></b> DCs staining positive for MPO, +/− 1 s.d., 4 ears per group, pooled from two independent experiments. (I) Representative histogram plots of MPO stained, RFP<b><sup>−</sup></b> (gray filled) and RFP<b><sup>+</sup></b> (black line) DCs. Mice were treated with control or neutrophil-depleting antibodies 1 d before i.d. challenge and the dermal cells were analyzed 14 days after infection.</p>