10.1371/journal.pone.0032193.g006 Georgina H. Cornish Georgina H. Cornish Sim L. Tung Sim L. Tung Daniel Marshall Daniel Marshall Steve Ley Steve Ley Benedict P. Seddon Benedict P. Seddon Activation of WT T cells in <i>Rag1</i><sup>−/−</sup><i>OX40<sup>Cre</sup> Ikbk2<sup>fx/fx</sup></i> mice. Public Library of Science 2013 wt cells 2013-02-20 08:44:28 Figure https://plos.figshare.com/articles/figure/_Activation_of_WT_T_cells_in_Rag1_8722_8722_OX40_Cre_Ikbk2_fx_fx_mice_/349580 <p>Eight week old <i>Rag1</i><sup>−/− </sup><i>OX40<sup>Cre</sup> Ikbk2<sup>fx/fx</sup></i> and <i>Rag1</i><sup>−/− </sup><i>OX40<sup>Cre</sup> Ikbk2<sup>fx/wt</sup></i> controls were reconstituted with 5×10<sup>7</sup> lymph node T cells from WT C57Bl6<sup>NIMR</sup> mice. Two weeks later, number and phenotype of transferred T cells in different recipients was determined. (A) Contour plots are of CD25 vs CD44 expression by CD4<sup>+</sup> TCR<sup>hi</sup> T cells (top row) and side scatter (SSC) vs CD44 by CD8<sup>+</sup> TCR<sup>hi</sup> T cells (bottom row) from dLN of <i>Rag1</i><sup>−/− </sup><i>OX40<sup>Cre</sup> Ikbk2<sup>fx/fx</sup></i> (<i>Ikbk2</i> fx/fx) and <i>Rag1</i><sup>−/− </sup><i>OX40<sup>Cre</sup> Ikbk2<sup>fx/wt</sup></i> (<i>Ikbk2</i> fx/wt) hosts. Numbers indicate % of cells in the adjacent gate. Data are representative of at least five mice per strain. (B) Scatter charts show absolute numbers of CD4<sup>+</sup> TCR<sup>hi</sup> or CD8<sup>+</sup> TCR<sup>hi</sup> T cells in skin draining lymph nodes (dLN), mesenteric lymph nodes (mLN) or spleen (SPN) in host <i>Rag1<sup>−/−</sup> OX40<sup>Cre</sup> Ikbk2<sup>fx/fx</sup></i> mice (fx/fx) or control <i>Rag1<sup>−/−</sup> OX40<sup>Cre</sup> Ikbk2<sup>fx/wt</sup></i> mice (fx/wt). Data are pool of two independent experiments.</p>