Expression of dicysteine mutants in Xenopus oocyte membranes.
Mike Mueckler
Carol Makepeace
10.1371/journal.pone.0031412.g001
https://plos.figshare.com/articles/figure/_Expression_of_dicysteine_mutants_in_Xenopus_oocyte_membranes_/349080
<p>Stage 5 <i>Xenopus</i> oocytes were injected with water (Sham) or with 50 ng of mRNA encoding the parental reporter construct (C-Tev) or the indicated dicysteine mutant. Two days post injection total oocyte membranes were prepared and subjected to immunoblot analysis using a rabbit polyclonal ab raised against a peptide corresponding to the C-terminal 15 residues of human Glut1.</p>
2013-02-20 08:41:54
dicysteine
mutants
xenopus
oocyte