Decrease in the absorbance of PEG<sub>40</sub>-CONH-(CH<sub>2</sub>)<sub>5</sub>-CONH-(CH<sub>2</sub>)<sub>6</sub>-NHCO-MTX with time during dialysis against 1mM HCl.
Itzik Cooper
Mati Fridkin
Yoram Shechter
10.1371/journal.pone.0158352.g001
https://plos.figshare.com/articles/figure/Decrease_in_the_absorbance_of_PEG_sub_40_sub_-CONH-_CH_sub_2_sub_sub_5_sub_-CONH-_CH_sub_2_sub_sub_6_sub_-NHCO-MTX_with_time_during_dialysis_against_1mM_HCl_/3484457
<p>PEG<sub>40</sub>-CONH-(CH<sub>2</sub>)<sub>5</sub>-CONH-(CH<sub>2</sub>)<sub>6</sub>-NHCO-MTX and PEG<sub>40</sub>-CONH-(CH<sub>2</sub>)<sub>6</sub>-NHCO-MTX (0.2 μmole/ml of each) were dialyzed against one liter of 1mM HCl (pH 3.0). At the indicated time points, 30 μl aliquots were withdrawn from the dialysis tube, diluted 20 folds and their absorbance at 305 nm was monitored. Data are presented as mean±STDEV (n = 3).</p>
2016-07-12 07:12:57
Carboxylate
acidic conditions
carboxylate
derivative
compound
release
NH 2
BSA
hydrolysis
conjugate
glutamate
MTX