Decrease in the absorbance of PEG<sub>40</sub>-CONH-(CH<sub>2</sub>)<sub>5</sub>-CONH-(CH<sub>2</sub>)<sub>6</sub>-NHCO-MTX with time during dialysis against 1mM HCl. Itzik Cooper Mati Fridkin Yoram Shechter 10.1371/journal.pone.0158352.g001 https://plos.figshare.com/articles/figure/Decrease_in_the_absorbance_of_PEG_sub_40_sub_-CONH-_CH_sub_2_sub_sub_5_sub_-CONH-_CH_sub_2_sub_sub_6_sub_-NHCO-MTX_with_time_during_dialysis_against_1mM_HCl_/3484457 <p>PEG<sub>40</sub>-CONH-(CH<sub>2</sub>)<sub>5</sub>-CONH-(CH<sub>2</sub>)<sub>6</sub>-NHCO-MTX and PEG<sub>40</sub>-CONH-(CH<sub>2</sub>)<sub>6</sub>-NHCO-MTX (0.2 μmole/ml of each) were dialyzed against one liter of 1mM HCl (pH 3.0). At the indicated time points, 30 μl aliquots were withdrawn from the dialysis tube, diluted 20 folds and their absorbance at 305 nm was monitored. Data are presented as mean±STDEV (n = 3).</p> 2016-07-12 07:12:57 Carboxylate acidic conditions carboxylate derivative compound release NH 2 BSA hydrolysis conjugate glutamate MTX