10.1371/journal.pone.0158599.g003 Niklas Lofruthe Niklas Lofruthe Inka Gallitz Inka Gallitz Lisa Traeger Lisa Traeger Nicole Bäumer Nicole Bäumer Isabell Schulze Isabell Schulze Tanja Kuhlmann Tanja Kuhlmann Carsten Müller-Tidow Carsten Müller-Tidow Andrea U. Steinbicker Andrea U. Steinbicker Hepatic mRNA levels of pSTAT3, MCP-1, SOD2 and activin B in the liver of WT mice after BA and intravenous iron injection. Public Library of Science 2016 iron deficiency anemia iron deficiency diet BA injection Inflammation Intravenous iron supplementation weight iron complexes saline 14 days IDA iron treatment AI Intravenous Iron Carboxymaltose 2016-07-12 07:11:52 Figure https://plos.figshare.com/articles/figure/Hepatic_mRNA_levels_of_pSTAT3_MCP-1_SOD2_and_activin_B_in_the_liver_of_WT_mice_after_BA_and_intravenous_iron_injection_/3484451 <p><b>(A)</b> Phosphorylated STAT3 levels compared to α-tubulin in the liver of C57BL/6 mice 4h after intraperitoneal BA or PBS administration followed by intravenous iron or PBS treatment for an additional 2h. <b>(B)</b> Hepatic MCP-1 mRNA levels were determined in WT mice after intraperitoneal BA or PBS administration followed by intravenous iron or PBS injection for an additional 2h (n = 3, 2-way ANOVA P<0,0001; **P = 0.007: PBS/PBS vs BA/PBS; *P = 0.02: PBS/PBS vs BA/iron; **P = 0.007: PBS/iron vs BA/PBS); (*P = 0.02: PBS/iron vs BA/iron; **P = 0.008: BA/PBS vs BA/iron; not shown in the graph). <b>(C)</b> Hepatic SOD2 mRNA levels in WT mice 4h after intraperitoneal BA or PBS administration followed by intravenous iron or PBS treatment for an additional 2h (n = 3, 2-way ANOVA P = 0.0002; **P = 0.004: PBS/PBS vs BA/PBS; **P = 0.004: PBS/PBS vs BA/iron); (**P = 0.006: PBS/iron vs BA/PBS; **P = 0.007: PBS/iron vs BA/iron, not shown in graph). <b>(D)</b> Hepatic activin B mRNA levels in WT mice 4h after intraperitoneal BA or PBS administration followed by intravenous iron or PBS injection for an additional 2h (n = 3, 2-way ANOVA P<0,0001; **P = 0.009: PBS/PBS vs BA/PBS; ***P = 0.0002: PBS/PBS vs BA/iron); (**P = 0.009: PBS/iron vs BA/PBS; ***P = 0.0003: PBS/iron vs BA/iron, not shown in graph).</p>