Gating strategy for the characterization of cellular infiltrates in the spleen and for the sorting of leukocyte subpopulations. Marta Matulova Hana Stepanova Frantisek Sisak Hana Havlickova Marcela Faldynova Kamila Kyrova Jiri Volf Ivan Rychlik 10.1371/journal.pone.0032346.g002 https://plos.figshare.com/articles/figure/_Gating_strategy_for_the_characterization_of_cellular_infiltrates_in_the_spleen_and_for_the_sorting_of_leukocyte_subpopulations_/346797 <p>The leukocytes were gated based on the CD45 expression (P1) and only CD45+ cells were included in the sorting and analyses. Sorted populations were followed: CD4 T-lymphocytes (P4+P3), CD8+ T-lymphocytes (P5), γδ T-lymphocytes (P6), monocytes/macrophages (P7), B-lymphocytes (P8) and heterophils (P9).</p> 2013-02-20 08:29:21 characterization cellular infiltrates spleen sorting leukocyte