10.1371/journal.ppat.1005717.g004
Rupkatha Mukhopadhyay
Rupkatha
Mukhopadhyay
Sujayita Roy
Sujayita
Roy
Rajkumar Venkatadri
Rajkumar
Venkatadri
Yu-Pin Su
Yu-Pin
Su
Wenjuan Ye
Wenjuan
Ye
Elena Barnaeva
Elena
Barnaeva
Lesley Mathews Griner
Lesley Mathews
Griner
Noel Southall
Noel
Southall
Xin Hu
Xin
Hu
Amy Q. Wang
Amy Q.
Wang
Xin Xu
Xin
Xu
Andrés E. Dulcey
Andrés E.
Dulcey
Juan J. Marugan
Juan J.
Marugan
Marc Ferrer
Marc
Ferrer
Ravit Arav-Boger
Ravit
Arav-Boger
The anti-HCMV activity of emetine is reduced in low-density HFFs.
Public Library of Science
2016
Human Cytomegalovirus Infection
Synergistic virus inhibition
RPS 14 knockdown
cell cycle regulation
EC
LOPAC
200. HCMV inhibition
interaction
emetine exploits RPS 14
RPS 14
MDM
DNA
CC
Low Dose Emetine
RPS 14 knockdown cells
processing S 14
MCMV
RPS 14 translocation
2016-06-23 07:04:29
Figure
https://plos.figshare.com/articles/figure/The_anti-HCMV_activity_of_emetine_is_reduced_in_low-density_HFFs_/3461396
<p><b>A)</b> Cells were seeded at indicated densities in a 96-well plate, infected with pp28-luciferase HCMV Towne (MOI = 1) and treated with emetine (75 nM) or GCV (5 μM). Luciferase activity was measured at 72 hpi and normalized to the activity of infected untreated cells. Data represent mean ± SE of triplicate determinations from a representative of two independent experiments. <b>B)</b> Same cell lysates as in A were collected for Western blotting at 72 hpi and expression of viral proteins UL44 and pp65 was determined. <b>C)</b> Supernatants from each well as in A were used to infect cells (1 million cells in a 96-well plate) in the corresponding well and luciferase activity was measured at 72 hpi. <b>D)</b> Cells were seeded into 12-well plates at 0.5 and 2 million cells/plate, infected with 100 PFU/well of HCMV Towne or MCMV, and treated with emetine (75 nM) or GCV (5 μM). After 10 days (for HCMV) or 3 days (for MCMV) plaques were stained with crystal violet and the number of plaques enumerated. Data shown are average of 2 wells (±SD) for a representative experiment from two different experiments.</p>