10.1371/journal.ppat.1005717.g004 Rupkatha Mukhopadhyay Rupkatha Mukhopadhyay Sujayita Roy Sujayita Roy Rajkumar Venkatadri Rajkumar Venkatadri Yu-Pin Su Yu-Pin Su Wenjuan Ye Wenjuan Ye Elena Barnaeva Elena Barnaeva Lesley Mathews Griner Lesley Mathews Griner Noel Southall Noel Southall Xin Hu Xin Hu Amy Q. Wang Amy Q. Wang Xin Xu Xin Xu Andrés E. Dulcey Andrés E. Dulcey Juan J. Marugan Juan J. Marugan Marc Ferrer Marc Ferrer Ravit Arav-Boger Ravit Arav-Boger The anti-HCMV activity of emetine is reduced in low-density HFFs. Public Library of Science 2016 Human Cytomegalovirus Infection Synergistic virus inhibition RPS 14 knockdown cell cycle regulation EC LOPAC 200. HCMV inhibition interaction emetine exploits RPS 14 RPS 14 MDM DNA CC Low Dose Emetine RPS 14 knockdown cells processing S 14 MCMV RPS 14 translocation 2016-06-23 07:04:29 Figure https://plos.figshare.com/articles/figure/The_anti-HCMV_activity_of_emetine_is_reduced_in_low-density_HFFs_/3461396 <p><b>A)</b> Cells were seeded at indicated densities in a 96-well plate, infected with pp28-luciferase HCMV Towne (MOI = 1) and treated with emetine (75 nM) or GCV (5 μM). Luciferase activity was measured at 72 hpi and normalized to the activity of infected untreated cells. Data represent mean ± SE of triplicate determinations from a representative of two independent experiments. <b>B)</b> Same cell lysates as in A were collected for Western blotting at 72 hpi and expression of viral proteins UL44 and pp65 was determined. <b>C)</b> Supernatants from each well as in A were used to infect cells (1 million cells in a 96-well plate) in the corresponding well and luciferase activity was measured at 72 hpi. <b>D)</b> Cells were seeded into 12-well plates at 0.5 and 2 million cells/plate, infected with 100 PFU/well of HCMV Towne or MCMV, and treated with emetine (75 nM) or GCV (5 μM). After 10 days (for HCMV) or 3 days (for MCMV) plaques were stained with crystal violet and the number of plaques enumerated. Data shown are average of 2 wells (±SD) for a representative experiment from two different experiments.</p>