The autoinhibited structure of <i>V</i>. <i>angustum</i> LuxO-RC is physiologically relevant. Hande Boyaci Tayyab Shah Amanda Hurley Bashkim Kokona Zhijie Li Christian Ventocilla Philip D. Jeffrey Martin F. Semmelhack Robert Fairman Bonnie L. Bassler Frederick M. Hughson 10.1371/journal.pbio.1002464.g003 https://plos.figshare.com/articles/figure/The_autoinhibited_structure_of_i_V_i_i_angustum_i_LuxO-RC_is_physiologically_relevant_/3403891 <p>Bioluminescence produced by a <i>V</i>. <i>cholerae</i> reporter strain carrying LuxO-controlled luciferase. Light production values for mutant strains are normalized to that produced by inactive, wild-type LuxO (WT). Mutations introduced into <i>V</i>. <i>cholerae</i> LuxO with the goal of disrupting the R-C interface display reduced bioluminescence—signifying increased LuxO activity—comparable to the constitutively active phosphomimetic LuxO D61E. The underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002464#pbio.1002464.s001" target="_blank">S1 Data</a>.</p> 2016-05-24 07:40:40 Vibrio cholerae virulence factor production ATPase superfamily LuxO activation Autoinducer binding inactivates autoinducers triggers repression receiver domains LuxO oligomer biofilm formation histidine sensor kinase family response regulator LuxO vibrio autoinducer molecules bind transmembrane receptors recapitulates elements AAA chemical signal molecules crystal structure autoinhibited state inhibitor molecule receiver domain phosphorylation