The autoinhibited structure of <i>V</i>. <i>angustum</i> LuxO-RC is physiologically relevant.
Hande Boyaci
Tayyab Shah
Amanda Hurley
Bashkim Kokona
Zhijie Li
Christian Ventocilla
Philip D. Jeffrey
Martin F. Semmelhack
Robert Fairman
Bonnie L. Bassler
Frederick M. Hughson
10.1371/journal.pbio.1002464.g003
https://plos.figshare.com/articles/figure/The_autoinhibited_structure_of_i_V_i_i_angustum_i_LuxO-RC_is_physiologically_relevant_/3403891
<p>Bioluminescence produced by a <i>V</i>. <i>cholerae</i> reporter strain carrying LuxO-controlled luciferase. Light production values for mutant strains are normalized to that produced by inactive, wild-type LuxO (WT). Mutations introduced into <i>V</i>. <i>cholerae</i> LuxO with the goal of disrupting the R-C interface display reduced bioluminescence—signifying increased LuxO activity—comparable to the constitutively active phosphomimetic LuxO D61E. The underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002464#pbio.1002464.s001" target="_blank">S1 Data</a>.</p>
2016-05-24 07:40:40
Vibrio cholerae
virulence factor production
ATPase superfamily
LuxO activation
Autoinducer binding inactivates
autoinducers triggers repression
receiver domains
LuxO oligomer
biofilm formation
histidine sensor kinase family
response regulator LuxO
vibrio autoinducer molecules bind
transmembrane receptors
recapitulates elements
AAA
chemical signal molecules
crystal structure
autoinhibited state
inhibitor molecule
receiver domain phosphorylation