Effect of HNF-1β ectopic expression on TGF-β1-induced phosphorylation of Smad3 in hRPTECs.
Mitsugu Omata
Yukiko Doke
Chikaomi Yamada
Kayoko Kawashima
Rumiko Sho
Kei Enomoto
Mayumi Furuya
Norio Inomata
10.1371/journal.pone.0154912.g005
https://plos.figshare.com/articles/figure/Effect_of_HNF-1_ectopic_expression_on_TGF-_1-induced_phosphorylation_of_Smad3_in_hRPTECs_/3391006
<p>Human RPTECs were stimulated with 3 ng/ml TGF-β1 for 48 h followed by re-stimulation with fresh TGF-β1 for 12 h or 24 h. After replacement with fresh TGF-β1, the hRPTECs were infected with 1.0 MOI and 2.0 MOI Ad-HNF1B or Ad-LacZ. Representative western blotting shows the expression of phosphorylated Smad3 and total Smad3 at 48 kD in Ad-HNF1B-transfected hRPTECs 12 (A) and 24 h (B) after TGF-β1 re-stimulation. Densitometric quantification of the corresponding bands was performed using an image analyzer. The data are presented after normalization to total Smad3 expression. Each column presents the means of twice independent experiments from non-stimulation with Ad-LacZ (white), TGF-β1 stimulation for 48 h followed by incubation with TGF-β1 and Ad-LacZ (hatched line), and TGF-β1 and Ad-HNF1B (dot) for 12 (C) and 24 h (D). Each dot symbol shows an individual value.</p>
2016-05-19 09:26:53
candidate molecule
profibrotic cytokine
validation studies
network analysis
extracellular matrix molecules
HNF
fibrosis model
Dedifferentiated Tubular Epithelial Cells Tubular epithelial cells
TEC
ureteral obstruction model
transcription factor hepatocyte
TGF
DNA microarray
fibrosis progression
ectopic expression