10.1371/journal.pone.0155803.g002 Alessandra Ghiani Alessandra Ghiani Nunzio D’Agostino Nunzio D’Agostino Sandra Citterio Sandra Citterio Assunta Raiola Assunta Raiola Riccardo Asero Riccardo Asero Amalia Barone Amalia Barone Maria Manuela Rigano Maria Manuela Rigano Putative allergens identified on immunoblots with extracts from fruits from M82, IL12-4 and IL7-3. Public Library of Science 2016 introgression lines harbour species Solanum pennellii genotype M 82. tomato allergens tomato line M 82 tomato line influences Cultivated Tomato Fruits Tomato IL allergenic 2016-05-16 08:01:36 Figure https://plos.figshare.com/articles/figure/Putative_allergens_identified_on_immunoblots_with_extracts_from_fruits_from_M82_IL12-4_and_IL7-3_/3387703 <p>(A) Protein extracts from three replicates of each line were analysed by immunoblotting using sera of tomato allergic subjects. Protein bands that showed a stronger reaction (black arrows) were eluted from the gels and identified using mass spectrometry. PG2A (Polygalacturonase 2A) Solyc10g080210; PME1 (Pectin methylesterase 1) Solyc07g064170; PME2.1 (Pectin methylesterase 2.1) Solyc07g064180; nsLTP 1 (non-specific lipid transfer protein type 1) Solyc10g075090. (B) Membranes were treated with periodic acid before immunoblotting with allergic patient sera. A 14 kDa protein (Profilin) detected in immunoblots is also shown.</p>