10.1371/journal.pone.0155803.g002
Alessandra Ghiani
Alessandra
Ghiani
Nunzio D’Agostino
Nunzio
D’Agostino
Sandra Citterio
Sandra
Citterio
Assunta Raiola
Assunta
Raiola
Riccardo Asero
Riccardo
Asero
Amalia Barone
Amalia
Barone
Maria Manuela Rigano
Maria
Manuela Rigano
Putative allergens identified on immunoblots with extracts from fruits from M82, IL12-4 and IL7-3.
Public Library of Science
2016
introgression lines harbour
species Solanum pennellii
genotype M 82.
tomato allergens
tomato line M 82
tomato line influences
Cultivated Tomato Fruits Tomato
IL
allergenic
2016-05-16 08:01:36
Figure
https://plos.figshare.com/articles/figure/Putative_allergens_identified_on_immunoblots_with_extracts_from_fruits_from_M82_IL12-4_and_IL7-3_/3387703
<p>(A) Protein extracts from three replicates of each line were analysed by immunoblotting using sera of tomato allergic subjects. Protein bands that showed a stronger reaction (black arrows) were eluted from the gels and identified using mass spectrometry. PG2A (Polygalacturonase 2A) Solyc10g080210; PME1 (Pectin methylesterase 1) Solyc07g064170; PME2.1 (Pectin methylesterase 2.1) Solyc07g064180; nsLTP 1 (non-specific lipid transfer protein type 1) Solyc10g075090. (B) Membranes were treated with periodic acid before immunoblotting with allergic patient sera. A 14 kDa protein (Profilin) detected in immunoblots is also shown.</p>