Recombinant FliC and truncated FliC expressed from plasmid pORI induce TNF-α in a TLR5-dependent manner.
Jesse D. Deere
W. L. William Chang
Luis D. Castillo
Kim A. Schmidt
Hung T. Kieu
Nicholas Renzette
Timothy Kowalik
Stephen W. Barthold
Barbara L. Shacklett
Peter A. Barry
Ellen E. Sparger
10.1371/journal.pone.0155629.g001
https://plos.figshare.com/articles/figure/Recombinant_FliC_and_truncated_FliC_expressed_from_plasmid_pORI_induce_TNF-_in_a_TLR5-dependent_manner_/3387202
<p>TNF-α ELISA of cell culture supernatants collected from TLR5-positive RAW424 (Blue) or its parental TLR5-negative RAW264.7 (Red) mouse macrophage cells. <b>(A)</b> RAW cells were treated with increasing concentrations of recombinant FliC. <b>(B)</b> Telomerized rhesus fibroblast cells were transiently transfected with plasmids containing full-length FliC (pORI<i>fliC</i>) or partially truncated FliCs (pORI<i>fliC</i>Δ196–378 or pORI<i>fliC</i>Δ141–398) and freeze thaw lysates were generated. Cell culture supernatants harvested from RAW cells treated with these lysates were assayed by an ELISA that measures TNF-α production. An average of two independent experiments and means with standard deviation are presented.</p>
2016-05-16 08:02:29
mouse macrophage cell line
Gag
fusion
Western blot analysis
macrophage cell line
codon optimized gag gene
immunodominant hypervariable domain
vaccine
rhesus macaques inoculated
novel RhCMV virus
AIDS
immunodeficiency virus
tumor necrosis factor
Nonhuman Primate Model
SIV p 27 capsid
Ongoing animals studies
TLR 5 adjuvant
protein
HIV
telomerized rhesus fibroblast
TNF