Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Manasi Das Sanjeeb K. Sahoo 10.1371/journal.pone.0032920.g008 https://plos.figshare.com/articles/figure/_Detection_of_early_induction_of_apoptosis_was_done_using_JC_1_dye_by_flow_cytometry_/335586 <p>Briefly, 1×10<sup>5</sup> cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.</p> 2012-03-21 01:33:06 induction apoptosis was done jc-1 dye