Overall rate of emigration (ORE) after intrathymic inoculation. Ana Rosa Pérez Luiz Ricardo Berbert Ailin Lepletier Silvia Revelli Oscar Bottasso Suse Dayse Silva-Barbosa Wilson Savino 10.1371/journal.pone.0034360.t002 https://plos.figshare.com/articles/dataset/_Overall_rate_of_emigration_ORE_after_intrathymic_inoculation_/333739 <p>Control and 14 days-infected animals were injected intrathymically with FITC or FITC plus TNF-α. Twenty-four hours after, thymuses, spleens and subcutaneous lymph nodes were screened for FITC<sup>+</sup> CD4/CD8-defined cell subsets and these data were adjusted in relation to the cellularity of each peripheral lymphoid organ and the percentage of thymus labelling, using the following formula: Overall rate of emigration (ORE) = (Absolute numbers of FITC<sup>+</sup> cells×cellularity in the peripheral lymphoid organ)/(% of FITC<sup>+</sup> cells in thymus). We verified an enlarged ORE values in infected animals, independently of whether they were inoculated or not with TNF-α. Data indicate that TNF-α promotes the output of immature DP<sup>+</sup> cells to both spleen and subcutaneous lymph nodes. Absolute numbers of FITC<sup>+</sup> cells, CD4<sup>+</sup>, CD8<sup>+</sup> and DN cells was expressed on 1.10<sup>4</sup> cells, while DP<sup>+</sup> cells was expressed on 1.10<sup>3</sup> cells. Cellularity of each peripheral lymphoid organ was expressed in 1.10<sup>6</sup> cells. Values are mean ± SEM of 5–6 mice/group (one representative experiment of two independent series). Difference between Control <i>versus T. cruzi</i> group = <sup>(</sup>*<sup>)</sup>p<0.05; <sup>(</sup>**<sup>)</sup>p<0.02. Differences between TNF-α treated <i>versus</i> not treated group = <sup>(Δ)</sup>p<0.05; <sup>(ΔΔ)</sup>p<0.02.</p> 2013-02-20 07:21:19 emigration intrathymic