10.1371/journal.pone.0033983.g001 Zhi-Yang Chang Zhi-Yang Chang Da-Wen Lu Da-Wen Lu Ming-Kung Yeh Ming-Kung Yeh Chiao-Hsi Chiang Chiao-Hsi Chiang Flatmount quantification of rat RGCs by image analysis software. Public Library of Science 2013 quantification rgcs 2013-02-20 07:21:18 Figure https://plos.figshare.com/articles/figure/_Flatmount_quantification_of_rat_RGCs_by_image_analysis_software_/333732 <p>Seven days post-treatment with intravitreal injection of vehicle (BSS as control) or NMDA solution. (A). Image of FG-labeled RGCs in flatmounted retina, bar = 100 µm. (B). Soma size distribution histograms. The histograms were generated by counting 10000 RGC cells and dividing into size groups with a 2 µm interval. RGC density was expressed as RGCs/mm<sup>2</sup> (mean ± SEM, n = 8). (C). RGC survival rates of different size groups after intravitreal NMDA (40 mM, 2 µL) treatment. Each point represents RGC survival rate (%) based on the individual RGC size population of control group, the dashed line represents total RGC survival rate (%).</p>