10.1371/journal.pone.0034377.g001
Aarthi Sundararajan
Aarthi
Sundararajan
Lifang Huan
Lifang
Huan
Katherine A. Richards
Katherine
A. Richards
Glendie Marcelin
Glendie
Marcelin
Shabnam Alam
Shabnam
Alam
HyeMee Joo
HyeMee
Joo
Hongmei Yang
Hongmei
Yang
Richard J. Webby
Richard
J. Webby
David J. Topham
David
J. Topham
Andrea J. Sant
Andrea
J. Sant
Mark Y. Sangster
Mark
Y. Sangster
The CD4 T cell response to NC infection.
Public Library of Science
2012
cd4
nc
2012-03-23 01:02:07
Figure
https://plos.figshare.com/articles/figure/_The_CD4_T_cell_response_to_NC_infection_/333727
<p>(A–D) Cytokine production by peptide-specific CD4 T cells. DR1 (A and B) and B10 (C and D) mice were infected intranasally with 40,000 EID<sub>50</sub> NC. Enriched CD4 T cells from the MedLN and spleen were analyzed on day 10 after infection. Frequencies of CD4 T cells secreting IL-2, IFN-γ, or IL-4 were determined by ELISpot assay after in vitro stimulation with antigen-presenting cells and individual 17-mer peptides. Peptide designations (x-axis) include the viral proteins of origin (HA, NA, NP, M1, and NS1). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034377#s3" target="_blank">Results</a> are normalized to spot counts per 10<sup>6</sup> CD4 T cells and are shown as the mean+SEM for 2–6 independent experiments for each peptide. Cells from at least three mice were pooled for each experiment. (E, F) Proportions of peptide-specific CD4 T cells secreting IL-2, IFN-γ, or IL-4. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034377#s3" target="_blank">Results</a> are compiled from the data shown in A–D and represent 4 (MedLN) or 6 (spleen) independent experiments evaluating 3–12 (MedLN) or 12–20 (spleen) individual peptides. The mean+SEM is shown.</p>