10.1371/journal.pone.0034377.g001 Aarthi Sundararajan Aarthi Sundararajan Lifang Huan Lifang Huan Katherine A. Richards Katherine A. Richards Glendie Marcelin Glendie Marcelin Shabnam Alam Shabnam Alam HyeMee Joo HyeMee Joo Hongmei Yang Hongmei Yang Richard J. Webby Richard J. Webby David J. Topham David J. Topham Andrea J. Sant Andrea J. Sant Mark Y. Sangster Mark Y. Sangster The CD4 T cell response to NC infection. Public Library of Science 2012 cd4 nc 2012-03-23 01:02:07 Figure https://plos.figshare.com/articles/figure/_The_CD4_T_cell_response_to_NC_infection_/333727 <p>(A–D) Cytokine production by peptide-specific CD4 T cells. DR1 (A and B) and B10 (C and D) mice were infected intranasally with 40,000 EID<sub>50</sub> NC. Enriched CD4 T cells from the MedLN and spleen were analyzed on day 10 after infection. Frequencies of CD4 T cells secreting IL-2, IFN-γ, or IL-4 were determined by ELISpot assay after in vitro stimulation with antigen-presenting cells and individual 17-mer peptides. Peptide designations (x-axis) include the viral proteins of origin (HA, NA, NP, M1, and NS1). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034377#s3" target="_blank">Results</a> are normalized to spot counts per 10<sup>6</sup> CD4 T cells and are shown as the mean+SEM for 2–6 independent experiments for each peptide. Cells from at least three mice were pooled for each experiment. (E, F) Proportions of peptide-specific CD4 T cells secreting IL-2, IFN-γ, or IL-4. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034377#s3" target="_blank">Results</a> are compiled from the data shown in A–D and represent 4 (MedLN) or 6 (spleen) independent experiments evaluating 3–12 (MedLN) or 12–20 (spleen) individual peptides. The mean+SEM is shown.</p>