10.1371/journal.pntd.0001583.g006
Bárbara Hissa
Bárbara
Hissa
Jacqueline G. Duarte
Jacqueline
G. Duarte
Ludmila F. Kelles
Ludmila
F. Kelles
Fabio P. Santos
Fabio
P. Santos
Helen L. del Puerto
Helen
L. del Puerto
Pedro H. Gazzinelli-Guimarães
Pedro
H. Gazzinelli-Guimarães
Ana M. de Paula
Ana
M. de Paula
Ubirajara Agero
Ubirajara
Agero
Oscar N. Mesquita
Oscar
N. Mesquita
Cristina Guatimosim
Cristina
Guatimosim
Egler Chiari
Egler
Chiari
Luciana O. Andrade
Luciana
O. Andrade
Cholesterol depletion leads to changes in lysosomal distribution within cells.
Public Library of Science
2012
microbiology
Molecular cell biology
depletion
leads
changes
lysosomal
2012-03-27 01:02:00
Figure
https://plos.figshare.com/articles/figure/_Cholesterol_depletion_leads_to_changes_in_lysosomal_distribution_within_cells_/333720
<p>Representative panels of lysosomal distribution in control cardiomyocyte (A) and cardiomyocytes pre-treated either with 10 mM (B) or 15 mM MβCD (C), 10 mM or (D) 15 mM HγCD (E), or 15 mM MβCD followed by 0.05 mM WSC (F). MβCD treated cardiomyocytes show significant changes in lysosomal dispersion in cell cytoplasm. After drug treatment, cells were washed and incubated with <i>T. cruzi</i> TCTs, M.O.I. of 50 parasites per cell for 40 minutes at 37°C. After cell invasion, cells were washed, fixed and immunostained for LAMP-1 (green) DAPI (blue) and analyzed under fluorescence microscope. In comparison to untreated cardiomyocytes (A), which exhibit homogenous and well distributed LAMP-1 labeling, MβCD treated cardiomyocytes, (B) and (C), show a heterogeneous LAMP-1 labeling with lysosomes localized predominantly near cell nuclei. On the other hand, HγCD treated cardiomyocytes and cholesterol-replenished cells, (E), (F) and (D), present lysosomal distribution similar to control cells. Scale bar: 10 µm.</p>