Figure 4 Nicolas Vabret Marc Bailly-Bechet Valérie Najburg Michaela Müller-Trutwin Bernard Verrier Frédéric Tangy 10.1371/journal.pone.0033502.g004 https://plos.figshare.com/articles/figure/_Figure_4_/322158 <p>. <b>Overlapping RNA fragments covering the entire genome of HIV-1 </b><b><i>hxb2</i></b><b>.</b> PCR fragments of approximately 500 bp, with overlaps of 250 bp, and covering the entire genome of HIV-1 <i>hxb2</i> were <i>in vitro</i> transcribed with T7 RNA polymerase into a set of uncapped and unpolyadenylated RNA fragments. After purification RNA fragments were migrated on Agilent-2100 Small RNA chips. A ladder on the left indicates the size in nucleotides.</p> 2012-04-18 00:35:58 immunology Infectious diseases microbiology molecular biology pathology