%0 Figure %A Jin, Mengyao %A Kumar, Anil %A Kumar, Santosh %D 2013 %T Effect of vitamin C on ethanol-induced CYP2A6 expression level and CYP2E1-mediated oxidative stress. %U https://plos.figshare.com/articles/figure/_Effect_of_vitamin_C_on_ethanol_induced_CYP2A6_expression_level_and_CYP2E1_mediated_oxidative_stress_/322150 %R 10.1371/journal.pone.0035505.g002 %2 https://plos.figshare.com/ndownloader/files/651661 %K ethanol-induced %K cyp2a6 %K cyp2e1-mediated %K oxidative %X

(A) CYP2A6 mRNA. (B) CYP2A6 protein. Both mRNA and protein expression levels were determined at 12 h in the presence of 100 mM ethanol treatment (−/+100 µM vitamin C 1 h prior to ethanol treatment). Blots are representative of at least three replicates. For each experiment, the mRNA/protein levels of various treatments were normalized relative to the untreated control, which has been set to 100%. (C) Representative figure of the determination of ROS levels at 2 h ethanol treatment with 0 and 100 µM vitamin C pretreatment for 1 h. The events (cell population) are presented in Y-axis and relative fluorescence intensity is presented in X-axis. (D) Bar graphs of MFI in the presence and absence of ethanol and vitamin C. The mean ± SD was calculated from at least triplicates and significance (p≤0.05; *) was determined using one-way ANOVA. The experiment was repeated three times.

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