10.1371/journal.pone.0036134.g002 Carine Villanueva Carine Villanueva Sandrine Jacquier Sandrine Jacquier Nicolas de Roux Nicolas de Roux DLK1 is expressed in specific nuclei of the hypothalamus. Public Library of Science 2012 nuclei 2012-04-26 02:02:50 Figure https://plos.figshare.com/articles/figure/_DLK1_is_expressed_in_specific_nuclei_of_the_hypothalamus_/317370 <p><b>A</b>) Immunohistochemistry using the C-19 antibody was performed on free-floating coronal brain sections from adult male mice as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036134#s2" target="_blank">materials and methods</a> (2,5× objective; Bregma −0.82 mm at the hypothalamic level according to the mouse brain atlas <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036134#pone.0036134-Franklin1" target="_blank">[21]</a>. B) Bregma −2.06 mm C) Higher magnification of the paraventricular nucleus (PVN), suprachiasmatic nucleus (SCN), supraoptic nucleus (SON) arcuate nucleus (ARC), and lateral hypothalamic nucleus (LH) (20× objective; scale bar: 80 µm).</p>